STUDIES OF THE TRANSMISSIBLE AGENT OF THE ROUS CHICKEN SARCOMA I PRECIPITATION WITH BASIC PROTEINS

Shemin , David; Sproul , E. E.; Jobling , James W.

doi:10.1084/jem.72.6.697

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Article| December 01 1940

STUDIES OF THE TRANSMISSIBLE AGENT OF THE ROUS CHICKEN SARCOMA I : PRECIPITATION WITH BASIC PROTEINS

David Shemin,

David Shemin

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

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E. E. Sproul,

E. E. Sproul

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

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James W. Jobling

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

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Author and Article Information

David Shemin

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

E. E. Sproul

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

James W. Jobling

From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York

Received: August 20 1940

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1940, by The Rockefeller Institute for Medical Research New York

1940

J Exp Med (1940) 72 (6): 697–705.

https://doi.org/10.1084/jem.72.6.697

The viscosity of Rous sarcoma extracts can be reduced with a polysaccharide enzyme isolated from pneumococcus or testis without destruction of the active agent and thus more concentrated active filtrates can be obtained.

The active agent can be precipitated with basic proteins.

The basic protein can be separated from the components originally coming from the tumor filtrate by electrophoresis.

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Copyright, 1940, by The Rockefeller Institute for Medical Research New York

1940

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