Monocytes are circulating precursors for tissue macrophages and dendritic cells (DCs) but are not recognized to directly participate in antigen presentation. We developed techniques to label mouse monocyte subsets with particulate tracers in vivo. Gr-1lo but not Gr-1hi monocytes were stably labeled by intravenous injection of 0.5-μm microspheres. Gr-1hi monocytes could be labeled when the microspheres were injected after systemic depletion of blood monocytes and spleen macrophages. In this condition, the phagocytic tracer was transferred to immature bone marrow monocytes by neutrophils and B cells that first carried the particles to the bone marrow. Moreover, antigens from B cells or proteins conjugated to the tracer particles were processed for presentation by monocytes and could induce T cell responses in the periphery. Cell-associated antigen taken up by bone marrow monocytes was retained intracellularly for presentation of the antigen days later when monocyte-derived DCs migrated to lymph nodes or in vitro after differentiation with granulocyte/macrophage colony-stimulating factor. These data reveal that immature monocytes unexpectedly sample antigen from the bone marrow environment and that they can present these antigens after they leave the bone marrow.
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20 March 2006
Article|
February 21 2006
Immature monocytes acquire antigens from other cells in the bone marrow and present them to T cells after maturing in the periphery
Frank Tacke,
Frank Tacke
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
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Florent Ginhoux,
Florent Ginhoux
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
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Claudia Jakubzick,
Claudia Jakubzick
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
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Nico van Rooijen,
Nico van Rooijen
2Department of Molecular Cell Biology, Free University Medical Center, 1081 HV Amsterdam, Netherlands
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Miriam Merad,
Miriam Merad
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
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Gwendalyn J. Randolph
Gwendalyn J. Randolph
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
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Frank Tacke
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
Florent Ginhoux
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
Claudia Jakubzick
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
Nico van Rooijen
2Department of Molecular Cell Biology, Free University Medical Center, 1081 HV Amsterdam, Netherlands
Miriam Merad
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
Gwendalyn J. Randolph
1Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029
CORRESPONDENCE Gwendalyn J. Randolph: [email protected]
Abbreviations used: CLL, clodronate-loaded liposome; FSC, forward scatter; LX, latex; PT, pertussis toxin.
Received:
October 20 2005
Accepted:
January 26 2006
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2006
J Exp Med (2006) 203 (3): 583–597.
Article history
Received:
October 20 2005
Accepted:
January 26 2006
Citation
Frank Tacke, Florent Ginhoux, Claudia Jakubzick, Nico van Rooijen, Miriam Merad, Gwendalyn J. Randolph; Immature monocytes acquire antigens from other cells in the bone marrow and present them to T cells after maturing in the periphery . J Exp Med 20 March 2006; 203 (3): 583–597. doi: https://doi.org/10.1084/jem.20052119
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