Bone marrow (BM)-derived professional antigen-presenting cells (pAPCs) are required for the generation of cytotoxic T lymphocyte (CTL) responses to vaccinia virus and poliovirus. Furthermore, these BM-derived pAPCs require a functional transporter associated with antigen presentation (TAP). In this report we analyze the requirements for BM-derived pAPCs and TAP in the initiation of CTL responses to lymphocytic choriomeningitis virus (LCMV) and influenza virus (Flu). Our results indicate a requirement for BM-derived pAPCs for the CTL responses to these viruses. However, we found that the generation of CTLs to one LCMV epitope (LCMV nucleoprotein 396–404) was dependent on BM-derived pAPCs but, surprisingly, TAP independent. The study of the CTL response to Flu confirmed the existence of this BM-derived pAPC-dependent/TAP-independent CTL response and indicated that the TAP-independent pathway is ∼10–300-fold less efficient than the TAP-dependent pathway.
Bone Marrow–Derived Antigen-Presenting Cells Are Required for the Generation of Cytotoxic T Lymphocyte Responses to Viruses and Use Transporter Associated with Antigen Presentation (Tap)-Dependent and -Independent Pathways of Antigen Presentation
Abbreviations used in this paper: B6, C57BL/6; B6D2, (C57BL/6 × DBA2)F1; BM, bone marrow; D2, DBA2; ER, endoplasmic reticulum; Flu, influenza virus; gp, glycoprotein; HAU, hemagglutination unit(s); LCMV, lymphocytic choriomeningitis virus; np, nucleoprotein; pAPC, professional APC; TAP, transporter associated with antigen presentation; TAP0/0, B6-Tap1tp 1Arp.
Luis J. Sigal, Kenneth L. Rock; Bone Marrow–Derived Antigen-Presenting Cells Are Required for the Generation of Cytotoxic T Lymphocyte Responses to Viruses and Use Transporter Associated with Antigen Presentation (Tap)-Dependent and -Independent Pathways of Antigen Presentation. J Exp Med 16 October 2000; 192 (8): 1143–1150. doi: https://doi.org/10.1084/jem.192.8.1143
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