Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells. In addition, recent evidence suggests that DCs may influence naive B cells during the initial priming of antibody responses. In this study, using three-color confocal microscopy and three-dimensional immunohistograms, we have observed that in the first few days after a primary immunization, cells with dendritic morphology progressively localize within primary B cell follicles. These cells were identified by their ability to bind a fusion protein consisting of the terminal cysteine-rich portion of the mouse mannose receptor and the Fc portion of human immunoglobulin (Ig)G1 (CR-Fc). In situ, these CR-Fc binding cells express major histocompatibility complex class II, sialoadhesin, and CD11c and are negative for other markers identifying the myeloid DC lineage, such as (CD11b), macrophages (F4/80), follicular DCs (FDC-M2), B cells (B220), and T cells (CD4). Using CR-Fc binding capacity and flow cytometry, the cells were purified from the draining lymph nodes of mice 24 h after immunization. When injected into naive mice, these cells were able to prime T cells as well as induce production of antigen-specific IgM and IgG1. Furthermore, they produced significantly more of the lymphocyte chemoattractant, macrophage inflammatory protein (MIP)-1α, than isolated interdigitating cells. Taken together, these results provide evidence that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes.
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20 September 1999
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September 20 1999
A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein
Claude Berney,
Claude Berney
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
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Suzanne Herren,
Suzanne Herren
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
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Christine A. Power,
Christine A. Power
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
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Siamon Gordon,
Siamon Gordon
bSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
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Luisa Martinez-Pomares,
Luisa Martinez-Pomares
bSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
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Marie H. Kosco-Vilbois
Marie H. Kosco-Vilbois
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
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Claude Berney
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
Suzanne Herren
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
Christine A. Power
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
Siamon Gordon
bSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
Luisa Martinez-Pomares
bSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
Marie H. Kosco-Vilbois
aSerono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
1used in this paper: BM-DC, bone marrow–derived DC; CR, cysteine-rich; 3D, three-dimension; DC, dendritic cell; FDC, follicular DC; MFI, mean fluorescence intensity; MIP, macrophage inflammatory protein; RANTES, regulated upon activation, normal T cell expressed and secreted
Received:
March 01 1999
Revision Requested:
July 12 1999
Accepted:
July 13 1999
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 1999 The Rockefeller University Press
1999
The Rockefeller University Press
J Exp Med (1999) 190 (6): 851–860.
Article history
Received:
March 01 1999
Revision Requested:
July 12 1999
Accepted:
July 13 1999
Citation
Claude Berney, Suzanne Herren, Christine A. Power, Siamon Gordon, Luisa Martinez-Pomares, Marie H. Kosco-Vilbois; A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein. J Exp Med 20 September 1999; 190 (6): 851–860. doi: https://doi.org/10.1084/jem.190.6.851
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