To determine the role of CD8+ T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8+ T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.
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15 March 1999
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March 15 1999
Dramatic Rise in Plasma Viremia after CD8+ T Cell Depletion in Simian Immunodeficiency Virus–infected Macaques
Xia Jin,
Xia Jin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Daniel E. Bauer,
Daniel E. Bauer
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Sarah E. Tuttleton,
Sarah E. Tuttleton
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Sharon Lewin,
Sharon Lewin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Agegnehu Gettie,
Agegnehu Gettie
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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James Blanchard,
James Blanchard
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Craig E. Irwin,
Craig E. Irwin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Jeffrey T. Safrit,
Jeffrey T. Safrit
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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John Mittler,
John Mittler
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Leor Weinberger,
Leor Weinberger
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Leondios G. Kostrikis,
Leondios G. Kostrikis
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Linqi Zhang,
Linqi Zhang
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Alan S. Perelson,
Alan S. Perelson
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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David D. Ho
David D. Ho
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Xia Jin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Daniel E. Bauer
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Sarah E. Tuttleton
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Sharon Lewin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Agegnehu Gettie
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
James Blanchard
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Craig E. Irwin
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Jeffrey T. Safrit
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
John Mittler
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Leor Weinberger
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Leondios G. Kostrikis
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Linqi Zhang
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Alan S. Perelson
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
David D. Ho
From *The Aaron Diamond AIDS Research Center, The Rockefeller University, New York 10016; ‡Tulane Regional Primate Research Center, Covington, Louisiana 70433; the §Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30329; and ‖Los Alamos National Laboratory, Los Alamos, New Mexico 87545
Address correspondence to David D. Ho, The Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Ave., New York, NY 10016. Phone: 212-448-5100; Fax: 212-725-1126; E-mail: [email protected]
S. Tuttleton is a student in the Tri-Institutional M.D.-Ph.D. Program supported by National Institutes of Health Medical Scientist Training Program grant GM 07739. This study was supported by National Institutes of Health grants AI40387, AI42848, AI43042, and AI38573, as well as by funding from the Bristol Myers Squibb Foundation and the Irene Diamond Fund.
Received:
December 22 1998
Revision Received:
January 19 1999
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (6): 991–998.
Article history
Received:
December 22 1998
Revision Received:
January 19 1999
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Citation
Xia Jin, Daniel E. Bauer, Sarah E. Tuttleton, Sharon Lewin, Agegnehu Gettie, James Blanchard, Craig E. Irwin, Jeffrey T. Safrit, John Mittler, Leor Weinberger, Leondios G. Kostrikis, Linqi Zhang, Alan S. Perelson, David D. Ho; Dramatic Rise in Plasma Viremia after CD8+ T Cell Depletion in Simian Immunodeficiency Virus–infected Macaques . J Exp Med 15 March 1999; 189 (6): 991–998. doi: https://doi.org/10.1084/jem.189.6.991
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