The requirement of β7 integrins for lymphocyte migration was examined during an ongoing immune response in vivo. Transgenic mice (OT-I) expressing an ovalbumin-specific major histocompatibility complex class I–restricted T cell receptor for antigen were rendered deficient in expression of all β7 integrins or only the αEβ7 integrin. To quantitate the relative use of β7 integrins in migration in vivo, equal numbers of OT-I and OT-I-β7−/− or OT-I-αE−/− lymph node (LN) cells were adoptively transferred to normal mice. Although OT-I-β7−/− LN cells migrated to mesenteric LN and peripheral LN as well as wild-type cells, β7 integrins were required for naive CD8 T cell and B cell migration to Peyer's patch. After infection with a recombinant virus (vesicular stomatitis virus) encoding ovalbumin, β7 integrins became critical for migration of activated CD8 T cells to the mesenteric LN and Peyer's patch. Naive CD8 T cells did not enter the lamina propria or the intestinal epithelium, and the majority of migration of activated CD8 T cells to the small and large intestinal mucosa, including the epithelium, was β7 integrin–mediated. The αEβ7 integrin appeared to play no role in migration during a primary CD8 T cell immune response in vivo. Furthermore, despite dramatic upregulation of αEβ7 by CD8 T cells after entry into the epithelium, long-term retention of intestinal intraepithelial lymphocytes was also αEβ7 independent.
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17 May 1999
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May 17 1999
The Role of β7 Integrins in CD8 T Cell Trafficking During an Antiviral Immune Response
Leo Lefrançois,
Leo Lefrançois
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Christina M. Parker,
Christina M. Parker
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Sara Olson,
Sara Olson
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Werner Muller,
Werner Muller
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Norbert Wagner,
Norbert Wagner
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Lynn Puddington
Lynn Puddington
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
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Leo Lefrançois
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Christina M. Parker
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Sara Olson
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Werner Muller
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Norbert Wagner
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Lynn Puddington
From the *Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the ‡Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the §Institute for Genetics, University of Cologne, 50937 Cologne, Germany
Address correspondence to Leo Lefrançois, UCONN Health Center, MC1310, Department of Medicine, 263 Farmington Ave., Farmington, CT 06030. Phone: 860-679-3242; Fax: 860-679-1287; E-mail: [email protected]
Received:
December 17 1998
Revision Received:
March 08 1999
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (10): 1631–1638.
Article history
Received:
December 17 1998
Revision Received:
March 08 1999
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Citation
Leo Lefrançois, Christina M. Parker, Sara Olson, Werner Muller, Norbert Wagner, Lynn Puddington; The Role of β7 Integrins in CD8 T Cell Trafficking During an Antiviral Immune Response . J Exp Med 17 May 1999; 189 (10): 1631–1638. doi: https://doi.org/10.1084/jem.189.10.1631
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