A major virulence factor in the stomach chronic infection by Helicobacter pylori is a protein toxin (VacA), which alters cell membrane trafficking of late endosomal/prelysosomal compartments. Its role in the chronic infection established by H. pylori is unknown. To test the possibility that VacA alters antigen processing taking place in prelysosomal compartments, we have used the well-established model of antigen processing and presentation consisting of tetanus toxoid–specific human (CD4+) T cells stimulated by autologous antigen-pulsed Epstein-Barr virus-transformed B cells. We found that VacA interferes with proteolytic processing of tetanus toxin and toxoid and specifically inhibits the Ii-dependent pathway of antigen presentation mediated by newly synthesized major histocompatibility complex (MHC) class II, while leaving unaffected the presentation pathway dependent on recycling MHC class II. The results presented here suggest that VacA may contribute to the persistence of H. pylori by interfering with protective immunity and that this toxin is a new useful tool in the study of the different pathways of antigen presentation.
Skip Nav Destination
Article navigation
5 January 1998
Brief Definitive Report|
January 05 1998
Selective Inhibition of Ii-dependent Antigen Presentation by Helicobacter pylori Toxin VacA
Maurizio Molinari,
Maurizio Molinari
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Mariolina Salio,
Mariolina Salio
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Carmela Galli,
Carmela Galli
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Nathalie Norais,
Nathalie Norais
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Rino Rappuoli,
Rino Rappuoli
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Antonio Lanzavecchia,
Antonio Lanzavecchia
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Cesare Montecucco
Cesare Montecucco
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Search for other works by this author on:
Maurizio Molinari
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Mariolina Salio
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Carmela Galli
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Nathalie Norais
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Rino Rappuoli
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Antonio Lanzavecchia
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Cesare Montecucco
From the *Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy; the ‡Basel Institute for Immunology, 4005 Basel, Switzerland; and the §Centro Ricerche IRIS, Chiron-Vaccines, 53100 Siena, Italy
Address correspondence to Cesare Montecucco, Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, Via G. Colombo 3, 35121 Padova, Italy. Phone: 39-49-827-6056; FAX: 39-49-827-6049; E-mail: [email protected]
M. Molinari and C. Galli wish to dedicate this work to the memory of Roland Graf.
Received:
October 08 1997
Revision Received:
October 29 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (1): 135–140.
Article history
Received:
October 08 1997
Revision Received:
October 29 1997
Citation
Maurizio Molinari, Mariolina Salio, Carmela Galli, Nathalie Norais, Rino Rappuoli, Antonio Lanzavecchia, Cesare Montecucco; Selective Inhibition of Ii-dependent Antigen Presentation by Helicobacter pylori Toxin VacA . J Exp Med 5 January 1998; 187 (1): 135–140. doi: https://doi.org/10.1084/jem.187.1.135
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement