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Xiaoyan Song
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Journal Articles
Jacco van Rheenen, Xiaoyan Song, Wies van Roosmalen, Michael Cammer, Xiaoming Chen, Vera DesMarais, Shu-Chin Yip, Jonathan M. Backer, Robert J. Eddy, John S. Condeelis
Journal:
Journal of Cell Biology
Journal of Cell Biology (2007) 179 (6): 1247–1259.
Published: 17 December 2007
Abstract
Lamellipodial protrusion and directional migration of carcinoma cells towards chemoattractants, such as epidermal growth factor (EGF), depend upon the spatial and temporal regulation of actin cytoskeleton by actin-binding proteins (ABPs). It is generally hypothesized that the activity of many ABPs are temporally and spatially regulated by PIP 2 ; however, this is mainly based on in vitro–binding and structural studies, and generally in vivo evidence is lacking. Here, we provide the first in vivo data that directly visualize the spatial and temporal regulation of cofilin by PIP 2 in living cells. We show that EGF induces a rapid loss of PIP 2 through PLC activity, resulting in a release and activation of a membrane-bound pool of cofilin. Upon release, we find that cofilin binds to and severs F-actin, which is coincident with actin polymerization and lamellipod formation. Moreover, our data provide evidence for how PLC is involved in the formation of protrusions in breast carcinoma cells during chemotaxis and metastasis towards EGF.
Includes: Supplementary data
Journal Articles
Ghassan Mouneimne, Lilian Soon, Vera DesMarais, Mazen Sidani, Xiaoyan Song, Shu-Chin Yip, Mousumi Ghosh, Robert Eddy, Jonathan M. Backer, John Condeelis
Journal:
Journal of Cell Biology
Journal of Cell Biology (2004) 166 (5): 697–708.
Published: 30 August 2004
Abstract
The epidermal growth factor (EGF)–induced increase in free barbed ends, resulting in actin polymerization at the leading edge of the lamellipodium in carcinoma cells, occurs as two transients: an early one at 1 min and a late one at 3 min. Our results reveal that phospholipase (PLC) is required for triggering the early barbed end transient. Phosphoinositide-3 kinase selectively regulates the late barbed end transient. Inhibition of PLC inhibits cofilin activity in cells during the early transient, delays the initiation of protrusions, and inhibits the ability of cells to sense a gradient of EGF. Suppression of cofilin, using either small interfering RNA silencing or function-blocking antibodies, selectively inhibits the early transient. Therefore, our results demonstrate that the early PLC and cofilin-dependent barbed end transient is required for the initiation of protrusions and is involved in setting the direction of cell movement in response to EGF.