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1-3 of 3
Marek Cyrklaff
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Journal Articles
Rubén Fernández-Busnadiego, Benoît Zuber, Ulrike Elisabeth Maurer, Marek Cyrklaff, Wolfgang Baumeister, Vladan Lučić
Journal:
Journal of Cell Biology
Journal of Cell Biology (2010) 188 (1): 145–156.
Published: 11 January 2010
Abstract
The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N -ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release.
Includes: Supplementary data
Journal Articles
Regina Rabl, Vincent Soubannier, Roland Scholz, Frank Vogel, Nadine Mendl, Andreja Vasiljev-Neumeyer, Christian Körner, Ravi Jagasia, Thomas Keil, Wolfgang Baumeister, Marek Cyrklaff, Walter Neupert, Andreas S. Reichert
Journal:
Journal of Cell Biology
Journal of Cell Biology (2009) 185 (6): 1047–1063.
Published: 15 June 2009
Abstract
Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized a mitochondrial membrane protein in yeast, Fcj1 (formation of CJ protein 1), which is specifically enriched in CJs. Cells lacking Fcj1 lack CJs, exhibit concentric stacks of inner membrane in the mitochondrial matrix, and show increased levels of F 1 F O –ATP synthase (F 1 F O ) supercomplexes. Overexpression of Fcj1 leads to increased CJ formation, branching of cristae, enlargement of CJ diameter, and reduced levels of F 1 F O supercomplexes. Impairment of F 1 F O oligomer formation by deletion of its subunits e / g (Su e / g ) causes CJ diameter enlargement and reduction of cristae tip numbers and promotes cristae branching. Fcj1 and Su e / g genetically interact. We propose a model in which the antagonism between Fcj1 and Su e / g locally modulates the F 1 F O oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristae tips.
Includes: Supplementary data
Journal Articles
Boyan K. Garvalov, Benoît Zuber, Cédric Bouchet-Marquis, Mikhail Kudryashev, Manuela Gruska, Martin Beck, Andrew Leis, Friedrich Frischknecht, Frank Bradke, Wolfgang Baumeister, Jacques Dubochet, Marek Cyrklaff
Journal:
Journal of Cell Biology
Journal of Cell Biology (2006) 174 (6): 759–765.
Published: 05 September 2006
Abstract
The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.
Includes: Supplementary data