Figure 1. Ian Collinson and coworkers identified two distinct nanotube types that mediate mitochondrial exchange between cells (1). Healthy cells with import-competent mitochondria were cocultured with import-defective cells expressing a More about this image found in Ian Collinson and coworkers identified two distinct nanotube types that med...

Figure 1. Meiotic phenotype of teo mutant males. (A–D) Cells shown in panels are from squashed testes fixed either in methanol/acetone (A–C) or with a formaldehyde-based technique (D), and stained for tubulin (green), Asl (red), and DNA (blue). More about this image found in Meiotic phenotype of teo mutant males. (A–D) Cells shown ...

Figure 2. Molecular and phenotypic characterization of mutations in teo. (A) Predicted protein products of teo mutant alleles. teo¹ carries a premature stop codon (Trp296Ter) due to a G>A substitution (c.887G>A). teo^M11 carries 2 deletions More about this image found in Molecular and phenotypic characterization of mutations in teo...

Figure 3. teo mutant males are defective in sperm individualization and have partially or completely empty seminal vesicles. (A and B) Preparations from squashed adult testes fixed with methanol/acetone and stained for DNA (blue), actin (red), More about this image found in teo mutant males are defective in sperm individualization ...

Figure 4. Teo-HA localization in adult Drosophila testes. (A) Whole-mount images of the apical parts of testes stained for HA (red), Bam (green), and DNA (blue). The top panels show epifluorescence images, while those on the bottom are optical More about this image found in Teo-HA localization in adult Drosophila testes. (A) Whole...

Figure 5. CycA and CycB downregulation rescues the teo mutant phenotype. (A) Average number of progeny (AP) from teo^M11 and teo^D9 mutant males in the absence (M11: teo^M11/teo^M11; +/+ and D9: teo^D9/teo^D9; +/+) or the presence of the CycA⁰³⁹⁴⁶ More about this image found in CycA and CycB downregulation rescues the teo mutant phenot...

Figure 6. CycA localization in wild-type and teo ^M11 /teo ^M11 adult testes. Upper panels: Whole-mount testes from wild-type and teo^M11/teo^M11 males stained for CycA showing very similar fusome structures. Bar, 50 µm. Lower panels: Wild-type More about this image found in CycA localization in wild-type and teo ^M11...

Figure 7. CycB localization in wild-type and teo ¹ /Df adult testes. (A and B) Spermatocytes and spermatids are from squashed adult testes of wild-type (A) and teo¹/Df (B) 4- to 6-day-old males maintained under a free-mating regime, fixed with More about this image found in CycB localization in wild-type and teo ¹...

Figure 8. Interactions of Teo with CycA, CycB, and APC/C components. (A and B) Representative WBs and quantitative analysis of CycB (A) and CycA (B) in testes from teo¹/Df, teo^M11/teo^M11, and teo^D9/teo^D9 mutants compared with wild-type testes; More about this image found in Interactions of Teo with CycA, CycB, and APC/C components. (A and B) Repre...

Figure 1. Sir3 levels and modifications do not impact telomere HC formation during the SP. (A) Left: Growth curve of S. cerevisiae grown in liquid glucose–based rich medium. Cells are first growing exponentially, fermenting glucose, and More about this image found in Sir3 levels and modifications do not impact telomere HC formation during th...

Figure 2. Molecular dynamics simulations suggest that dissociation from the nuclear periphery is sufficient to drive telomere HC formation. (A) Main structural elements implemented in the model, namely, the spindle pole body, the chromosomes, More about this image found in Molecular dynamics simulations suggest that dissociation from the nuclear p...

Figure 3. Esc1 is the main active anchor a few hours after glucose exhaustion and prevents telomere HC formation in the center of the nucleus. (A) Representative fluorescent images of Rap1-GFP strains during the exponential phase, and at 16 h More about this image found in Esc1 is the main active anchor a few hours after glucose exhaustion and pre...

Figure 4. Esc1-independent anchoring is regulated by the Ras/PKA pathway. (A–D) Representative fluorescent images of the telomere-associated protein Rap1 tagged with GFP. The scale bar is 2 µm. Quantifications of telomere clustering: dot plots More about this image found in Esc1-independent anchoring is regulated by the Ras/PKA pathway. (A–D) Repr...

Figure 5. Esc1 prevents telomere HC formation in NQ cells. (A) Representative fluorescent images of Rap1-GFP WT (yAT2487) and esc1Δ (yAT3811) strains during the exponential phase, at 24 h and 48 h, and during the SP. The scale bar is 2 µm. More about this image found in Esc1 prevents telomere HC formation in NQ cells. (A) Representative fluore...

Figure 6. Mutation of Esc1 serine 1450 to alanine phenocopies ESC1 deletion. (A) Schematic representation of Sir4 and Esc1 conserved domains according to Faure et al. (2019) and Deshpande et al. (2019) . The gray zone represents the Sir4/Esc1 More about this image found in Mutation of Esc1 serine 1450 to alanine phenocopies ESC1 d...

Figure 7. Schematic representation of a nucleus undergoing metabolic transitions in WT and esc1Δ cells. Esc1-P denotes phosphorylated Esc1. Diagram of nuclear transitions in WT and esc1 cells. The diagram is divided into two main sections: More about this image found in Schematic representation of a nucleus undergoing metabolic transitions in W...