Figure 1. LAD organization is compromised in LMNA-knockdown hiPSC-CMs. (A) Experimental workflow of hiPSC-CMs from seeding, introduction of siScr and LMNA siRNA (siScr or siLMNA), time point, and collection for downstream analyses. (B) More about this image found in LAD organization is compromised in LMNA-knockdown hiPSC-CM...

Figure 2. LMNA reduction compromises peripheral chromatin spatial positioning. (A) Representative LMNB1 CUT&RUN tracks (z-score) in siScr (gray) and siLMNA (blue) hiPSC-CMs; yellow shading indicates locations of two LMNA-sensitive probes. More about this image found in LMNA reduction compromises peripheral chromatin spatial positioning. (A) R...

Figure 3. The LINC complex contributes to aberrant chromatin organization in LMNA-knockdown cells. (A) Schematic showing DNKASH disruption of cytoskeleton and nuclear lamina connection by replacing endogenous nesprin proteins (green). (B) More about this image found in The LINC complex contributes to aberrant chromatin organization in ...

Figure 4. Severing cytoskeleton-lamina connections in LMNA-knockdown cells preserves a subset of LAD peripheral positioning. (A) Representative LMNB1 CUT&RUN tracks (z-score) in siScr mCh (gray), siLMNA mCh (blue), and siLMNA DNKASH (purple) More about this image found in Severing cytoskeleton-lamina connections in LMNA-knockdown...

Figure 5. Microtubules control peripheral chromatin positioning via the LINC complex in LMNA-knockdown cells. (A) Schematic showing paclitaxel (stabilization) and colchicine (depolymerization) effect on microtubule structure. (B) More about this image found in Microtubules control peripheral chromatin positioning via the LINC complex ...

Figure 6. The cytoskeleton contributes to aberrant LAD organization in LMNA-knockdown cells. hiPSC-CMs have different groups of LADs with varying sensitivities to cytoskeletal inputs upon nuclear lamina disruption. LADs with lower LMNB1 More about this image found in The cytoskeleton contributes to aberrant LAD organization in LMNA...

Figure 1. SC structure and protein components across model organisms. (A) Schematic of the SC architecture showing the lateral elements (blue), which associate with chromatin (gray), connected by the SC central region comprising transverse More about this image found in SC structure and protein components across model organisms. (A) Schematic ...

Figure 2. Meiotic chromosome axis assembly and recombination control. (A) Assembly of the meiotic chromosome axis. Meiosis-specific cohesin complexes (light blue rings) extrude chromatin into loops and provide the structural foundation for More about this image found in Meiotic chromosome axis assembly and recombination control. (A) Assembly o...

Figure 3. SC architecture in mouse and C. elegans. (A) Schematic representation of the mouse SC region. The molecular organization of SC central region components and AlphaFold 3–predicted structure of the SYCE–SIX6OS1 complex are shown on the More about this image found in SC architecture in mouse and C. elegans. (A) Schematic re...

Figure 4. SC morphogenesis is tightly linked to steps in homologous recombination. (A) Temporal coordination of SC morphogenesis through meiotic prophase I. Meiosis-specific cohesins load in pre-meiotic S phase and organize chromatin into arrays More about this image found in SC morphogenesis is tightly linked to steps in homologous recombination. (A...

Figure 5. Models of crossover patterning. (A) Coarsening model. HEI10 (magenta) initially localizes uniformly along the SC. Through diffusion-mediated coarsening, HEI10 accumulates at a subset of sites at the expense of neighboring foci, driving More about this image found in Models of crossover patterning. (A) Coarsening model. HEI10 (magenta) init...

Figure 1. An electron micrograph of in vitro reconstitution of the Golgi complex (from Rabouille et al., J. Cell Biol. 129:605–618, 1995) . An electron micrograph of the reconstituted Golgi complex. The micrograph displays reconstituted Golgi More about this image found in An electron micrograph of in vitro reconstitution of the Golgi complex (fro...

Figure 2. Catherine (image taken in 2024) . A photo of Catherine. A photo of Catherine taken on a sunny day in 2024. More about this image found in Catherine (image taken in 2024) . A photo of Catherine. A photo of Cath...