Figure 1. Centromeres segregate within a narrow time window but not with perfect synchrony. (A) Schematic depicting cohesin cleavage and subsequent sister chromatid separation; cohesin complexes in orange. (B) Fluorescent labeling of More about this image found in Centromeres segregate within a narrow time window but not with perfect sync...

Figure 2. Separase activity has a larger influence on chromosome segregation synchrony than microtubule dynamics. (A, C, and E) Frequency distributions and Gaussian fit (continuous lines) of the time difference between the separation of More about this image found in Separase activity has a larger influence on chromosome segregation synchron...

Figure 3. Separase-mediated feedback on securin degradation is unlikely. (A) Frequency distribution and Gaussian fit (continuous lines) of the time difference between the separation of centromeres 1 and 2 after expression of nondegradable cyclin More about this image found in Separase-mediated feedback on securin degradation is unlikely. (A) Frequen...

Figure 4. Separase autoactivation is unlikely. (A) Schematic depicting a positive feedback loop, where separase accelerates its own activation downstream of securin degradation. (B) Computational model for separase (Sep) release mediated by More about this image found in Separase autoactivation is unlikely. (A) Schematic depicting a positive fe...

Figure 5. Small-number effects cause stochasticity in sister chromatid separation time. (A) Schematic of the stochastic model of separase-mediated cohesin removal. Separase activation (through securin degradation) is represented by a constant More about this image found in Small-number effects cause stochasticity in sister chromatid separation tim...

Figure 1. A second wave of postnatal villus SMC expansion in the later postnatal period. (A) αSMA+ star cells (arrowheads) at the villus tip are located near villus blood capillaries. Staining for αSMA+ villus SMCs (cyan) and blood capillaries More about this image found in A second wave of postnatal villus SMC expansion in the later postnatal peri...

Figure 2. Eosinophil postnatal recruitment pattern and cellular interactome suggest a role in villus SMC development. (A) Villus stroma is smaller in the absence of eosinophils. Staining for fibronectin (red, top) and tenascin C (green, bottom) More about this image found in Eosinophil postnatal recruitment pattern and cellular interactome suggest a...

Figure 3. Eosinophils promote postnatal villus SMC expansion and differentiation. (A) Eosinophils are associated with villus SMCs in adult mice. Staining for eosinophils (green, SIGLECF) and villus SMCs (cyan, αSMA) in Balb/c and ΔdblGata1 adult More about this image found in Eosinophils promote postnatal villus SMC expansion and differentiation. (A)...

Figure 4. Intestinal eosinophils preferentially express Tgfb2 and promote a myogenic gene expression program in gut fibroblasts. (A) CD22+ eosinophils are at the leading edge of villus SMC network expansion. Staining for CD22 (green), SIGLECF More about this image found in Intestinal eosinophils preferentially express Tgfb2 and pr...

Figure 5. TGFβ and intestinal eosinophils promote conversion of Pdgfrb+ fibroblasts to villus SMCs. (A) Experimental outline of pup treatment with control or TGFβ-blocking antibodies. (B–E) TGFβ signaling blockade inhibits postnatal villus SMC More about this image found in TGFβ and intestinal eosinophils promote conversion of Pdgfrb...

Figure 1. Cortactin is degraded in PCVs in response to inflammatory stimuli that induce neutrophil recruitment. (A) Representative 3D confocal images (Leica SP8) of cremasteric PCVs stained for CD31 (magenta) and cortactin (green) (Scale bars = More about this image found in Cortactin is degraded in PCVs in response to inflammatory stimuli that indu...

Figure 2. Neutrophils induce endothelial cortactin degradation in vivo and in vitro. (A and B) Representative 3D-confocal images (Nikon A1) of cremaster muscles stimulated with TNFα for 4 h and stained for CD31 (magenta), MRP-14 (cyan), and More about this image found in Neutrophils induce endothelial cortactin degradation in vivo...

Figure 3. Endothelial cortactin is degraded by NSP. (A and B) Flow cytometric quantification of cortactin MFI in HUVEC with NSP inhibition. Neutrophils (1 × 10⁶/ml) were treated with the NE inhibitor Siv, the general serine protease inhibitors More about this image found in Endothelial cortactin is degraded by NSP. (A and B) Flow cytometric quanti...

Figure 4. Neutrophils deliver CatG into EC in vivo and in vitro. (A) Representative 3D super-resolution confocal images (Zeiss Airyscan) of HUVEC incubated with cell-free neutrophil supernatant (cf-NS) of non-stimulated (ice control) or More about this image found in Neutrophils deliver CatG into EC in vivo and in vi...