The cell membrane of the unicellular algae Distigma proteus is associated with arrays of parallel microtubules. Fragments of the membrane-microtubule complex have been isolated and partially purified. The microtubules were stable in vitro at room temperature as well as at 0 degree C, but were specifically and rapidly disassembled by Ca2+. After removal of all endogenous microtubules, the membrane-microtubule complex could be reassembled from brain microtubule protein and denuded Distigma membrane fragments. The readded microtubules bound in a fixed orientation, and only to those regions of membrane that are normally associated with microtubules in vivo.
Article| April 01 1984
Disassembly and reconstitution of a membrane-microtubule complex.
J M Murray
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1984) 98 (4): 1481–1487.
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J M Murray; Disassembly and reconstitution of a membrane-microtubule complex.. J Cell Biol 1 April 1984; 98 (4): 1481–1487. doi: https://doi.org/10.1083/jcb.98.4.1481
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