When L929 cells are exposed to 5 μg/ml dexamethasone, synthesis of a 90,000 M(r) polypeptide is induced within 12 h. Flattening of the cells begins at about this time and progresses to become quite prominent after 48 h of exposure. Two-dimensional PAGE and partial proteolytic fingerprints identify the 90,000 M(r) polypeptide as gelsolin, a Ca(++)-dependent inhibitor of actin polymerization. Thus, this system provides evidence that gelsolin may have a role in regulating cell shape in response to physiological agents such as glucocorticoids.
Article| February 01 1983
Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1983) 96 (2): 577–581.
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KW Lanks, EJ Kasambalides; Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells . J Cell Biol 1 February 1983; 96 (2): 577–581. doi: https://doi.org/10.1083/jcb.96.2.577
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