Trifluoperazine, a drug that binds to Ca2+-calmodulin and inhibits its interaction with other proteins, was found to inhibit growth and phagocytosis in a macrophagelike cell line, J774.16. Both effects were reversible and occurred at the same concentrations of drug (25--50 microM) that inhibited the activation of cyclic nucleotide phosphodiesterase by calmodulin in vitro. Fc-mediated phagocytosis was also depressed by W-7, a sulfonamide derivative that inhibits the activity of Ca2+-calmodulin. In contrast, taxol, a drug that stabilizes cellular microtubules, had no effect on Fc-mediated phagocytosis although it inhibited cell growth at nanomolar concentrations. The inhibitory effects of trifluoperazine and W-7 on phagocytosis suggest that calmodulin may be involved in this complex cellular function.
Trifluoperazine inhibits phagocytosis in a macrophagelike cultured cell line.
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S B Horwitz, G H Chia, C Harracksingh, S Orlow, S Pifko-Hirst, J Schneck, L Sorbara, M Speaker, E W Wilk, O M Rosen; Trifluoperazine inhibits phagocytosis in a macrophagelike cultured cell line.. J Cell Biol 1 December 1981; 91 (3): 798–802. doi: https://doi.org/10.1083/jcb.91.3.798
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