The half-life of a gap-junction polypeptide band migrating at 21,000 Mr on SDS polyacrylamide gels isolated from mouse liver is measured to be 5 h. Two low-molecular wight bands, probably related to the 21,000 Mr material by proteolysis, have measured half-lives of 4.6 and 5.2 h. Gap junctions are labeled in vivo using the 14C-bicarbonate labeling procedure, followed by quantitative fluorography.
Article| August 01 1981
Five-hour half-life of mouse liver gap-junction protein.
R F Fallon
D A Goodenough
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1981) 90 (2): 521–526.
R F Fallon, D A Goodenough; Five-hour half-life of mouse liver gap-junction protein.. J Cell Biol 1 August 1981; 90 (2): 521–526. doi: https://doi.org/10.1083/jcb.90.2.521
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