Using rhodamine 123 to stain mitochondria of the human cell line HL-60, we have followed their increase over the cell cycle by flow cytometry. A near-linear synthesis of mitochondrial mass was shown to occur over the cell cycle. A comparison with the cell's DNA synthesis pattern obtained by the same technique established a common time-base. The mitochondrial synthesis curve changes with culture age. As a control, thd dye was tested for its binding specificity and for its use to resolve mitochondria microscopically. Its stoichiometric range was established and, above 0.25 microgram/ml, it was shown to reduce growth rate and cell viability in culture.
Article| May 01 1981
Proliferation of mitochondria during the cell cycle of the human cell line (HL-60).
T W James
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1981) 89 (2): 256–260.
T W James, R Bohman; Proliferation of mitochondria during the cell cycle of the human cell line (HL-60).. J Cell Biol 1 May 1981; 89 (2): 256–260. doi: https://doi.org/10.1083/jcb.89.2.256
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