Binding of 125I-labeled insulin to the surface receptors of Cloudman S-91 mouse melanoma cells (CCL 53.1) was studied at various phases (M, G1, S, and G2) in the cell cycle. Insulin-binding activity was persistently present during the cell cycle but the highest activity was noted at the S-phase. The insulin once bound to the cell surface receptors at any phase of the cell cycle was internalized and degraded, presumably through a lysosomal pathway. Insulin-indexing activity of melanoma cells was not affected by melanocyte-stimulating hormone.
Article| January 01 1981
Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
B B Fuller
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1981) 88 (1): 241–244.
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N Shimizu, Y Shimizu, B B Fuller; Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.. J Cell Biol 1 January 1981; 88 (1): 241–244. doi: https://doi.org/10.1083/jcb.88.1.241
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