Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron microscopy of such dispersed nuclear fibers has shown a beads-on-a-string configuration for these four types of sperm chromatin. These structures are morphologically similar to those described in somatic cell nuclei as nucleosomes, although in sperm chromatin the basic proteins associated with DNA were significantly different from histones.
Article| October 01 1980
Electron microscope evidence for the presence of globular structures in different sperm chromatins.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1980) 87 (1): 280–284.
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M Gusse, P Chevaillier; Electron microscope evidence for the presence of globular structures in different sperm chromatins.. J Cell Biol 1 October 1980; 87 (1): 280–284. doi: https://doi.org/10.1083/jcb.87.1.280
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