Intracellular recordings of cultured mouse thioglycolate-induced peritoneal exudate macrophages reveal that these cells can exhibit two different types of electrophysiological properties characterized by differences in their current-voltage relationships and their resting membrane potentials. The majority of cells had low resting membrane potentials (-20 to -40 mV) and displayed current-voltage relationships that were linear for inward-going current pulses and rectifying for outward-going pulses. Small depolarizing transients, occurring either spontaneously or induced by current pulses, were seen in some cells with low resting membrane potentials. A second smaller group of cells exhibited more hyperpolarized resting membrane potentials (-60 to -90 mV) and S-shaped current-voltage relationships associated with a high-resistance transitional region. Cells with S-shaped current-voltage relationships sometimes exhibited two stable states of membrane potential on either side of the high-resistance transitional region. These data indicate that macrophages exhibit complex electrophysiological properties often associated with excitable cells.
Article| April 01 1980
Nonlinear current-voltage relationships in cultured macrophages.
E K Gallin
D R Livengood
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1980) 85 (1): 160–165.
E K Gallin, D R Livengood; Nonlinear current-voltage relationships in cultured macrophages.. J Cell Biol 1 April 1980; 85 (1): 160–165. doi: https://doi.org/10.1083/jcb.85.1.160
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