Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.
Article| October 01 1979
High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.
D A Fischman
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1979) 83 (1): 249–254.
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W Ip, D A Fischman; High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.. J Cell Biol 1 October 1979; 83 (1): 249–254. doi: https://doi.org/10.1083/jcb.83.1.249
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