The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.
Article| April 01 1979
Myosin synthesis in embryonic chicken fibroblasts.
R B Young
W G Bergen
P B Blauwiekel
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1979) 81 (1): 115–122.
R B Young, W G Bergen, P B Blauwiekel; Myosin synthesis in embryonic chicken fibroblasts.. J Cell Biol 1 April 1979; 81 (1): 115–122. doi: https://doi.org/10.1083/jcb.81.1.115
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