When suspensions of Halobacterium halobium are exposed to bright light, the light-scattering properties of the bacteria change. This light-scattering response can produce a transmission decrease of about 1% throughout the red and near-infrared region. The action spectrum for the light-scattering response appropriately matches the absorption spectrum of bacteriorhodopsin. The response is eliminated by cyanide p-trifluoro-methoxyphenylhydrazone, a proton ionophore, and by triphenylmethylphosphonium, a membrane permanent cation. A mild hypertonic shock induces a similar light-scattering change, suggesting that bright light causes the bacteria to shrink about 1% in volume, thereby producing the light-scattering response.
Article| December 01 1978
Bacteriorhodopsin induces a light-scattering change in Halobacterium halobium.
C L Wey
P L Ahl
R A Cone
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1978) 79 (3): 657–662.
C L Wey, P L Ahl, R A Cone; Bacteriorhodopsin induces a light-scattering change in Halobacterium halobium.. J Cell Biol 1 December 1978; 79 (3): 657–662. doi: https://doi.org/10.1083/jcb.79.3.657
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