A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
Article| October 01 1978
Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.
W S Adair
U W Goodenough
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1978) 79 (1): 281–285.
W S Adair, D Jurivich, U W Goodenough; Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.. J Cell Biol 1 October 1978; 79 (1): 281–285. doi: https://doi.org/10.1083/jcb.79.1.281
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