The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block.
Article| September 01 1978
A new method for excitation-contraction uncoupling in frog skeletal muscle.
J del Castillo
G Escalona de Motta
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1978) 78 (3): 782–784.
J del Castillo, G Escalona de Motta; A new method for excitation-contraction uncoupling in frog skeletal muscle.. J Cell Biol 1 September 1978; 78 (3): 782–784. doi: https://doi.org/10.1083/jcb.78.3.782
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