Nuclei within myotubes do not synthesize DNA for replication. Accordingly, cultures of myotubes display low levels of DNA polymerase activity. The coincidental decline in DNA polymerase activity and increased formation of multinucleated myotubes during culture does not prove that the loss of capacity to synthesize DNA is a consequence of fusion. Tne experiments described demonstrate that myogenic cells prevented from fusing have low levels of DNA polymerase activity. This is consistent with the notion that, in myogenic cultures, there is a population of mononucleated cells, the myoblasts, which have withdrawn from the mitotic cycle before fusion.
Article| April 01 1978
DNA polymerase activity in muscle cultures
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1978) 77 (1): 99–102.
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GC Yeoh, D Greenstein, H Holtzer; DNA polymerase activity in muscle cultures. J Cell Biol 1 April 1978; 77 (1): 99–102. doi: https://doi.org/10.1083/jcb.77.1.99
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