The participation of hepatic Golgi apparatus in the intracellular transport of blood-destined proteins has been analyzed using Golgi fractions enriched in cis and trans components of the Golgi apparatus. SDS-polyacrylamide gel electrophoresis of the liver Golgi fractions showed several proteins corresponding in relative proportions and mobilities with serum proteins. After a pulse injection of labeled leucine, the secretory content of the cis Golgi fraction was labeled earlier than the trans Golgi fraction. Taken together, the results show the participation of the liver Golgi apparatus in the secretion of most of the serum proteins and provide documentation for a sequential progression of secretory protein through the cis and trans components of the Golgi apparatus.
Article|
January 01 1978
Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.
J J Bergeron
D Borts
J Cruz
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1978) 76 (1): 87–97.
Citation
J J Bergeron, D Borts, J Cruz; Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.. J Cell Biol 1 January 1978; 76 (1): 87–97. doi: https://doi.org/10.1083/jcb.76.1.87
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