Bacteriophage immunoassays, radioimmunoassays, and biological assays have been used to measure levels of NGF in media conditioned by rat C-6 glioma cells in culture. By all three criteria, these cells secrete a macromolecule which is indistinguishable from mouse submandibular gland NGF.
Secretion of nerve growth factor by central nervous system glioma cells in culture.
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R A Murphy, J Oger, J D Saide, M H Blanchard, B G Arnason, C Hogan, N J Pantazis; Secretion of nerve growth factor by central nervous system glioma cells in culture.. J Cell Biol 1 March 1977; 72 (3): 769–773. doi: https://doi.org/10.1083/jcb.72.3.769
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