Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded by tubocurarine or atropine were given into the third ventricle. 2. 12, or 24 h after injection the brains were prepared for autoradiography. Injections of alpha-bungarotoxin (radioinert) in buffer, or of [3H]parathyroid hormone in buffer, served as controls. The various patterns of labeling suggest the presence of nicotinic-cholinergic neurons within the arcuate and basolateral regions of the hypothalamus including the supraoptic and suprachiasmatic nuclei and, in addition, the central nucleus of the amygdala.
An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection.
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J Silver, R B Billiar; An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection.. J Cell Biol 1 December 1976; 71 (3): 956–963. doi: https://doi.org/10.1083/jcb.71.3.956
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