In sections of human dentine (carious and sound) and bone examined with the electron microscope, apatite crystallites were seen to present long thin profiles somewhat suggestive of a cylindrical shape, broad profiles indicative of a plate-like shape, and profiles intermediate between these two extremes. With a special stereoscopic specimen holder allowing the specimen to be tilted through an angle of 30° it was possible to record images of two profiles of the same crystallite from different angles and thus gain information concerning the 3-dimensional morphology of crystallites showing a thin profile. In all fields so examined, the thin-profile crystallites that were properly oriented with respect to the axis of tilt exhibited a different width dimension in each of the two micrographs. From this it is concluded that the thin profiles actually represented edge views of plate-like crystallites.
Electron Microscopic Observations on the Three-Dimensional Morphology of Apatite Crystallites of Human Dentine and Bone'
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Erling Johansen, Harold F. Parks; Electron Microscopic Observations on the Three-Dimensional Morphology of Apatite Crystallites of Human Dentine and Bone' . J Biophys and Biochem Cytol 1 July 1960; 7 (4): 743–746. doi: https://doi.org/10.1083/jcb.7.4.743
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