In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The advantage of adding fixative to the bottom of the gradient and of using en bloc staining with uranyl acetate is also demonstrated.
Article| December 01 1975
Isolation of liver lysosomes by iron loading. Ultrastructural characterization.
J L Ericsson
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 67 (3): 887–894.
H Glaumann, H Jansson, B Arborgh, J L Ericsson; Isolation of liver lysosomes by iron loading. Ultrastructural characterization.. J Cell Biol 1 December 1975; 67 (3): 887–894. doi: https://doi.org/10.1083/jcb.67.3.887
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