The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
Article| September 01 1975
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
F G Moser
B P Dorman
F H Ruddle
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 66 (3): 676–680.
F G Moser, B P Dorman, F H Ruddle; Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.. J Cell Biol 1 September 1975; 66 (3): 676–680. doi: https://doi.org/10.1083/jcb.66.3.676
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