Colchicine, vinblastine, and vincristine inhibit the mitogenic stimulation of lymphocytes by concanavalin A as measured by the incorporation of [3H]thymidine and the appearance of blast cells. The inhibitory effect of colchicine could not be accounted for by diminution in cell viability or by metaphase arrest of mitosis in the stimulated cells. Moreover, the inhibition of [3H]thymidine incorporation was not due to blockage of thymidine transport or inhibition of DNA synthesis inasmuch as addition of colchicine had no effect on cells in the S phase of the cell cycle. The time of inhibition was correlated with the kinetics of cellular commitment to lectin activation and the kinetic data indicated that colchicine blocks stimulation early in the sequence of events following addition of the mitogen. These findings support the hypothesis that cytoplasmic microtubular function plays a role in the commitment of resting cells to undergo mitotic division.
Article| July 01 1975
Inhibition by colchicine of the mitogenic stimulation of lymphocytes prior to the S phase.
J L Wang
G R Gunther
G M Edelman
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 66 (1): 128–144.
J L Wang, G R Gunther, G M Edelman; Inhibition by colchicine of the mitogenic stimulation of lymphocytes prior to the S phase.. J Cell Biol 1 July 1975; 66 (1): 128–144. doi: https://doi.org/10.1083/jcb.66.1.128
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