An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers seen with phase-contrast optics. High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibody show a periodic fluorescence. Measurements indicate that the lengths of the fluorescent segments are variable with an average of 1.2 mum while the spacing between segments is approximately 0.4 mum.
Article| June 01 1975
Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1975) 65 (3): 549–561.
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E Lazarides; Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.. J Cell Biol 1 June 1975; 65 (3): 549–561. doi: https://doi.org/10.1083/jcb.65.3.549
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