Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
Article| February 01 1975
Two improved methods for preparing ferritin-protein conjugates for electron microscopy.
B R Olsen
R A Berg
D J Prockop
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 64 (2): 331–339.
Y Kishida, B R Olsen, R A Berg, D J Prockop; Two improved methods for preparing ferritin-protein conjugates for electron microscopy.. J Cell Biol 1 February 1975; 64 (2): 331–339. doi: https://doi.org/10.1083/jcb.64.2.331
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