Low resistance junctions between axons of crayfish ganglia are studied by freeze-fracture and negative staining. In freeze-fracture, fracture planes that go through a junctional membrane expose two faces, both internal, called face A and face B. Face A belongs to the internal membrane leaflet and faces the gap. Face B belongs to the external membrane leaflet and faces the axoplasm. Face A displays pits, 60–100 Å in diameter, arranged in a hexagonal array with a unit cell of ∼200 Å. An ∼25 Å bump is frequently seen at the center of each pit. Some pits are occupied by a globule ∼125 Å in diameter, which displays a central depression ∼25 Å in size. Face B contains globules also arranged in a fairly regular hexagonal pattern. The center-to-center distance between adjacent globules is most frequently ∼200 Å; however, occasionally certain globules are seen separated by a distance as short as ∼125 Å. The top surface of the globules occasionally displays a starlike profile and seems to contain a central depression ∼25 Å in diameter. In negatively stained preparations of membranes from the nerve cord, two types of membranes are seen containing a fairly regular pattern. In one, globules ∼95 Å in diameter form a hexagonal close packing with a unit cell of ∼95 Å. In the other, globules of the same size are organized in a larger hexagonal array with a unit cell of ∼155 Å (swollen arrangement). Some of the globules forming the swollen arrangement are seen containing six subunits. The six subunits form a hexagon which is skewed with respect to the main rows of hexagons in such a way that the subunits lie on rows which make an angle of ∼37° with the main rows.
LOW RESISTANCE JUNCTIONS IN CRAYFISH : II. Structural Details and Further Evidence for Intercellular Channels by Freeze-Fracture and Negative Staining
Camillo Peracchia; LOW RESISTANCE JUNCTIONS IN CRAYFISH : II. Structural Details and Further Evidence for Intercellular Channels by Freeze-Fracture and Negative Staining . J Cell Biol 1 April 1973; 57 (1): 66–76. doi: https://doi.org/10.1083/jcb.57.1.66
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