Separation of Neurospora mitochondrial outer membranes from the inner membrane/matrix fraction was effected by digitonin treatment and discontinuous density gradient centrifugation. The solubilization of four isoleucine-valine biosynthetic enzymes was studied as a function of digitonin concentration and time of incubation in the detergent. The kinetics of the appearance of valine biosynthetic function in fractions outside of the inner membrane/matrix fraction, coupled with enzyme solubilization patterns similar to that for the matrix marker, mitochondrial malate dehydrogenase, indicate that the four isoleucine-valine pathway enzymes are localized in the mitochondrial matrix.
SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA : II. Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway
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W. E. Cassady, E. H. Leiter, A. Bergquist, R. P. Wagner; SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA : II. Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway . J Cell Biol 1 April 1972; 53 (1): 66–72. doi: https://doi.org/10.1083/jcb.53.1.66
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