The synthesis of mitochondrial DNA (mDNA) in HeLa cells is selectively inhibited by relatively low concentrations of ethidium bromide. After exposure of cells to strongly inhibitory concentrations of the drug, the apparent superhelix density of mDNA is rapidly increased, as judged by its buoyant density in CsCl in the presence of ethidium bromide. Mitochondrial DNA synthesized in the presence of partially inhibitory concentrations of ethidium bromide is also altered in its buoyant density in the presence of the dye, but is more heterogeneous in this respect. However, the change in buoyant density of newly synthesized mDNA may be explained by changes in structure other than a change in superhelix density, as indicated by its increased resistance to digestion by pancreatic DNase.
Article| October 01 1971
THE EFFECT OF ETHIDIUM BROMIDE ON MITOCHONDRIAL DNA SYNTHESIS AND MITOCHONDRIAL DNA STRUCTURE IN HELA CELLS
Robert D. Leibowitz
From the Massachusetts Institute of Technology, Department of Biology, Cambridge, Massachusetts 02139.
Dr. Leibowitz's present address is the Department of Biological Sciences, Columbia University, New York 10027.
Received: January 15 1971
Revision Received: March 24 1971
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1971 by The Rockefeller University Press
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Robert D. Leibowitz; THE EFFECT OF ETHIDIUM BROMIDE ON MITOCHONDRIAL DNA SYNTHESIS AND MITOCHONDRIAL DNA STRUCTURE IN HELA CELLS . J Cell Biol 1 October 1971; 51 (1): 116–122. doi: https://doi.org/10.1083/jcb.51.1.116
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