The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.
LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
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K. E. Kuettner, R. Eisenstein, L. W. Soble, C. Arsenis; LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization . J Cell Biol 1 May 1971; 49 (2): 450–458. doi: https://doi.org/10.1083/jcb.49.2.450
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