Sequential changes occurring in the etioplasts of the primary leaf of 7-day-old dark-grown barley seedlings upon continuous illumination with 20 lux have been investigated by electron microscopy, in vivo spectrophotometry, and thin-layer chromatography. Following photoconversion of the protochlorophyllide pigment to chlorophyllide and the structural transformation of the crystalline prolamellar bodies, the tubules of the prolamellar bodies are dispersed into the primary lamellar layers. As both chlorophyll a and b accumulate, extensive formation of grana takes place. After 4 hr of greening, protochlorophyllide starts to reaccumulate, and concomitantly both large and small crystalline prolamellar bodies are formed. This protochlorophyllide is rapidly photoconverted upon exposure of the leaves to high light intensity, which also effects a rapid reorganization of the recrystallized prolamellar bodies into primary lamellar layers.
Article|
February 01 1970
MACROMOLECULAR PHYSIOLOGY OF PLASTIDS : VIII. Pigment and Membrane Formation in Plastids of Barley Greening under Low Light Intensity
K. W. Henningsen,
K. W. Henningsen
From the Institute of Genetics, University of Copenhagen, Copenhagen, 1353, Denmark.
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J. E. Boynton
J. E. Boynton
From the Institute of Genetics, University of Copenhagen, Copenhagen, 1353, Denmark.
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K. W. Henningsen
From the Institute of Genetics, University of Copenhagen, Copenhagen, 1353, Denmark.
J. E. Boynton
From the Institute of Genetics, University of Copenhagen, Copenhagen, 1353, Denmark.
Dr. Boynton's present address is Department of Botany, Duke University, Durham, North Carolina 27706
Received:
June 04 1969
Revision Received:
September 16 1969
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1970 by The Rockefeller University Press
1970
J Cell Biol (1970) 44 (2): 290–304.
Article history
Received:
June 04 1969
Revision Received:
September 16 1969
Citation
K. W. Henningsen, J. E. Boynton; MACROMOLECULAR PHYSIOLOGY OF PLASTIDS : VIII. Pigment and Membrane Formation in Plastids of Barley Greening under Low Light Intensity . J Cell Biol 1 February 1970; 44 (2): 290–304. doi: https://doi.org/10.1083/jcb.44.2.290
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