The base composition of DNA was determined for individual chromosomes from the dipteran Chironomus tentans and for each one of six different segments of one of the chromosomes. The isolations were carried out by micromanipulation and the DNA purines were first extracted from the isolated components and afterwards separated by means of microelectrophoresis on a cellulose fiber. It was found that DNA from this material has an unusual composition corresponding to a guanine + cytosine content of about 30%. This composition was not a function of the polytenic condition but was also found for DNA from testis tissue. Furthermore Drosophila has a more traditional base composition for the bulk of DNA. Statistically significant variations in base data were found between whole chromosomes as well as between the segments from one of the chromosomes.
Article|
May 01 1969
THE DNA BASE COMPOSITION OF INDIVIDUAL CHROMOSOMES AND CHROMOSOME SEGMENTS FROM CHIRONOMUS TENTANS
B. Daneholt
,
B. Daneholt
From the Department of Histology, Karolinska Institutet, Stockholm, Sweden
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J.-E. Edström
J.-E. Edström
From the Department of Histology, Karolinska Institutet, Stockholm, Sweden
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B. Daneholt
From the Department of Histology, Karolinska Institutet, Stockholm, Sweden
J.-E. Edström
From the Department of Histology, Karolinska Institutet, Stockholm, Sweden
Received:
December 27 1967
Revision Received:
January 09 1969
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1969 by The Rockefeller University Press.
1969
J Cell Biol (1969) 41 (2): 620–624.
Article history
Received:
December 27 1967
Revision Received:
January 09 1969
Citation
B. Daneholt, J.-E. Edström; THE DNA BASE COMPOSITION OF INDIVIDUAL CHROMOSOMES AND CHROMOSOME SEGMENTS FROM CHIRONOMUS TENTANS . J Cell Biol 1 May 1969; 41 (2): 620–624. doi: https://doi.org/10.1083/jcb.41.2.620
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