Tradescantia pollen grains, isolated during the period of DNA synthesis in the generative cell, accumulate deoxythymidine triphosphate (dTTP)-3H after incubation with thymidine-3H in the presence of millimolar deoxyadenosine. Most of this dTTP-3H was found to resist extraction by the fixative, cold ethanol-acetic acid, and its location was investigated by radioautography with thin, dry emulsion. Substantial binding of dTTP-3H occurred as an artifact; but when nuclei were isolated from the fixed pollen grains by sonication, it was found that they were differentially labeled: generative nuclei contained dTTP-3H, vegetative nuclei did not. This observation is discussed and is interpreted as evidence supporting the idea that thymidine is phosphorylated only in the generative cell of the pollen grain.
Article| September 01 1968
RADIOAUTOGRAPHIC LOCALIZATION OF DEOXYTHYMIDINE TRIPHOSPHATE IN TRADESCANTIA POLLEN GRAINS DURING DNA SYNTHESIS
S. T. Takats
From the Department of Biology, Temple University, Philadelphia, Pennsylvania 19122
Received: March 05 1968
Revision Received: April 30 1968
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1968 by The Rockefeller University Press.
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S. T. Takats; RADIOAUTOGRAPHIC LOCALIZATION OF DEOXYTHYMIDINE TRIPHOSPHATE IN TRADESCANTIA POLLEN GRAINS DURING DNA SYNTHESIS . J Cell Biol 1 September 1968; 38 (3): 509–514. doi: https://doi.org/10.1083/jcb.38.3.509
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