Segments cut from growing oat coleoptiles and pea stems were fed glucose-3H in presence and absence of the growth hormone indoleacetic acid (IAA). By means of electron microscope radioautography it was demonstrated that new cell wall material is deposited both at the wall surface (apposition) and within the preexisting wall structure (internally). Quantitative profiles for the distribution of incorporation with position through the thickness of the wall were obtained for the thick outer wall of epidermal cells. With both oat coleoptile and pea stem epidermal outer walls, it was found that a larger proportion of the newly synthesized wall material appeared to become incorporated within the wall in the presence of IAA. Extraction experiments on coleoptile tissue showed that activity that had been incorporated into the cell wall interior represented noncellulosic constituents, mainly hemicelluloses, whereas cellulose was deposited largely or entirely by apposition. It seems possible that internal incorporation of hemicelluloses plays a role in the cell wall expansion process that is involved in cell growth.
Article| December 01 1967
RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS
Peter M. Ray
From the Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee and the Division of Natural Sciences, University of California, Santa Cruz, California 95060.
Received: March 08 1967
Revision Received: August 07 1967
Accepted: August 07 1967
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1967 by The Rockefeller University Press
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Peter M. Ray; RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS . J Cell Biol 1 December 1967; 35 (3): 659–674. doi: https://doi.org/10.1083/jcb.35.3.659
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