A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effects; (c) the fractions are collected continuously, with size fractionation being evidenced; and (d) particles are separated into fractions of higher and lower activities as compared with the control population.
FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
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Lester Packer, Park S. Nobel, Elizabeth L. Gross, Howard C. Mel; FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS . J Cell Biol 1 March 1966; 28 (3): 443–448. doi: https://doi.org/10.1083/jcb.28.3.443
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