The cell population kinetics of excised, cultured pea roots was studied with the use of tritiated thymidine and colchicine to determine (1) the influence of excision, (2) the influence of sucrose concentration, (3) the average mitotic cycle duration, and (4) the duration of mitosis and the G1, S, and G2 periods of interphase.1 The results indicate that the process of excision causes a drop in the frequency of mitotic figures when performed either at the beginning of the culture period or after 100 hours in culture. This initial decrease in frequency of cell division is independent of sucrose concentration, but the subsequent rise in frequency of division, after 12 hours in culture, is dependent upon sucrose concentration. Two per cent sucrose maintains the shortest mitotic cycle duration. The use of colchicine indicated an average cycle duration of 20 hours, whereas the use of tritiated thymidine produced an average cycle duration of 17 hours.
Article| October 01 1965
CELL POPULATION KINETICS OF EXCISED ROOTS OF PISUM SATIVUM
Jack Van't Hof
From the Biology Department, Brookhaven National Laboratory, Upton, New York.
Dr. Van't Hof's present address is Department of Botany, University of Minnesota, Minneapolis
Received: April 16 1965
Online Issn: 1540-8140
Print Issn: 0021-9525
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Jack Van't Hof; CELL POPULATION KINETICS OF EXCISED ROOTS OF PISUM SATIVUM . J Cell Biol 1 October 1965; 27 (1): 179–189. doi: https://doi.org/10.1083/jcb.27.1.179
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