Correction: Chemical transformation of the multibudding yeast, Aureobasidium pullulans

To make competent cells, a single A. pullulans colony was inoculated into 5 ml of YPD (4% glucose) and grown overnight (∼18 h) at 24°C with agitation. The following day, the overnight culture was diluted to a final density of ∼5 × 10⁶ cells/ml in 50 ml of YPD (4% glucose) in a 250-ml flask. Cells were grown at 22–24°C with agitation at 200 rpm for ∼2–3 h until reaching a density of ∼10⁷ cells/ml, unless indicated otherwise. Cells were harvested via centrifugation at 2,254 rcf for 8 min in 50 ml conical tubes and then resuspended in 1 ml sterile room-temperature deionized water and transferred to a 1.5 ml Eppendorf tube before pelleting at 9,391 rcf for 10 s. The cells were rinsed once in 500-µl sterile Competence Buffer (10 mM Tris pH 8, 1 mM EDTA, 100 mM LiOAc, 1 M sorbitol) and pelleted at 9,391 rcf for 10 s. Finally, cells were resuspended at a final density of ∼2 × 10⁹ cells/ml in Competence Buffer, usually ∼250 µl, and divided into aliquots in 1.5 ml Eppendorf tubes with ∼10⁸ cells in each aliquot (∼60–65 µl in each). For conditions with amino acids supplemented into the Competence Buffer, Complete Supplement Mixture minus uracil (1004-100; Sunrise Science Products) and uracil (600-621-9; Sigma-Aldrich) were added to the Competence Buffer at a final concentration of 0.96 and 0.025 g/l, respectively. This corresponds to an amino acid concentration 1.25× the concentration used in a standard complete synthetic medium previously shown to improve the transformation of S. cerevisiae (Yu et al., 2016). Competence Buffer with amino acids was prepared fresh just before use and filter-sterilized. To make frozen competent cells for future use, aliquots of cells in Competence Buffer were transferred to a −80°C freezer.