Vol. 191, No. 7, December 27, 2010. Pages .
After publication of the paper, the authors discovered an error in figure preparation. In Fig. 3 B of the original manuscript, the authors used the same mlc-11 GFP channel kymograph in both the mlc1-11 (left) and iqg1Δ (right) panels. The authors have provided the corrected image and the affected legends (Fig. 3 and Video 2). This error in figure preparation does not impact the conclusions of the paper.
Myo1 targeting during cytokinesis depends on Mlc1 and Iqg1. (A) myo1-TD2 localization depends on Mlc1 and Iqg1. Wild-type (WT) (XDY154), mlc1-11 (XDY173), and iqg1Δ (XDY218) cells carrying plasmid pRS316-MYO1-TD2-GFP were grown in SC-Ura medium at 23°C and then examined for myo1-TD2 localization. (B) Full-length Myo1 localization depends on Mlc1 and Iqg1. Strains YEF6179 (mlc1-11 MYO1-GFP CDC3-RFP; left) and YEF6325 (iqg1Δ CDC3-RFP, pRS316-MYO1-C-GFP; right) were grown in liquid SC-Leu media at 23°C and then imaged by 3D dual-color time-lapse microscopy at 23°C with indicated intervals. Montage images of the GFP, RFP, and merged channels from the representative time-lapse data are shown here. Arrowheads indicate the splitting of septin hourglass into two cortical rings, which coincides with mitotic exit and the onset of cytokinesis. (C) myo1-TD2 localization in cells containing different iqg1 alleles. Plasmid pRS315 derivatives carrying the indicated iqg1 alleles (see Table III) were transformed into strain XDY218. Transformants were grown in SC-Ura-Leu medium at 23°C and then quantified for myo1-TD2 localization. APC, APC/C recognition site; CHD, calponin-homology domain; IQ, IQ motifs; Ras-GAP, RasGAP-related domain; RasGAP-C, RasGAP C terminus–related domain.
Myo1 targeting during cytokinesis depends on Mlc1 and Iqg1. (A) myo1-TD2 localization depends on Mlc1 and Iqg1. Wild-type (WT) (XDY154), mlc1-11 (XDY173), and iqg1Δ (XDY218) cells carrying plasmid pRS316-MYO1-TD2-GFP were grown in SC-Ura medium at 23°C and then examined for myo1-TD2 localization. (B) Full-length Myo1 localization depends on Mlc1 and Iqg1. Strains YEF6179 (mlc1-11 MYO1-GFP CDC3-RFP; left) and YEF6325 (iqg1Δ CDC3-RFP, pRS316-MYO1-C-GFP; right) were grown in liquid SC-Leu media at 23°C and then imaged by 3D dual-color time-lapse microscopy at 23°C with indicated intervals. Montage images of the GFP, RFP, and merged channels from the representative time-lapse data are shown here. Arrowheads indicate the splitting of septin hourglass into two cortical rings, which coincides with mitotic exit and the onset of cytokinesis. (C) myo1-TD2 localization in cells containing different iqg1 alleles. Plasmid pRS315 derivatives carrying the indicated iqg1 alleles (see Table III) were transformed into strain XDY218. Transformants were grown in SC-Ura-Leu medium at 23°C and then quantified for myo1-TD2 localization. APC, APC/C recognition site; CHD, calponin-homology domain; IQ, IQ motifs; Ras-GAP, RasGAP-related domain; RasGAP-C, RasGAP C terminus–related domain.
Full-length Myo1 disappears from the bud neck at the onset of cytokinesis in mlc1-11 and iqg1Δ cells. (Left) Strain: YEF6179 (a mlc1-11 MYO1-GFP CDC3-mCherry:LEU2). Green, Myo1-GFP; red, Cdc3-RFP. 1-min time-lapse interval is shown. (Right) Strain: YEF6325 (a iqg1Δ CDC3-mCherry:LEU2, pRS316-MYO1-C-GFP). Green, Myo1-GFP; red, Cdc3-RFP. 1.5-min time-lapse interval is shown.
Full-length Myo1 disappears from the bud neck at the onset of cytokinesis in mlc1-11 and iqg1Δ cells. (Left) Strain: YEF6179 (a mlc1-11 MYO1-GFP CDC3-mCherry:LEU2). Green, Myo1-GFP; red, Cdc3-RFP. 1-min time-lapse interval is shown. (Right) Strain: YEF6325 (a iqg1Δ CDC3-mCherry:LEU2, pRS316-MYO1-C-GFP). Green, Myo1-GFP; red, Cdc3-RFP. 1.5-min time-lapse interval is shown.
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