Caspase-14 is a unique member of the evolutionarily conserved family of cysteinyl aspartate–specific proteinases, which are mainly involved in inflammation and apoptosis. However, recent evidence also implicates these proteases in proliferation and differentiation. Although most caspases are ubiquitously expressed, caspase-14 expression is confined mainly to cornifying epithelia, such as the skin. Moreover, caspase-14 activation correlates with cornification, indicating that it plays a role in terminal keratinocyte differentiation. The determination of in vitro conditions for caspase-14 activity paved the way to identifying its substrates. The recent development of caspase-14–deficient mice underscored its importance in the correct degradation of (pro)filaggrin and in the formation of the epidermal barrier that protects against dehydration and UVB radiation. Here, we review the current knowledge on caspase-14 in skin homeostasis and disease.
The skin is the largest organ of the body and protects the organism against external physical, chemical, and biological insults such as wounding, UVB radiation, and microorganisms. It also provides a water-impermeable barrier that prevents dehydration. This major barrier resides in the upper layers of the epidermis (for review see Segre, 2006). The epidermis is the upper part of the skin that is continuously renewed. The basal layer, or stratum basale, of the epidermis contains proliferating keratinocytes (Fig. 1).
Upon withdrawal from the cell cycle, these basal keratinocytes detach from the basement membrane and undergo a terminal differentiation program to become corneocytes in the outer layers of the epidermis. This process is called cornification. In the intermediate stratum spinosum, the cells reinforce their cytoskeletal keratin filament network, and adjacent cells interact via many desmosomes, a specialized type of cell junction, to resist physical trauma. In the stratum granulosum, the keratinocytes become more flattened and express certain proteins such as profilaggrin and loricrin, which aggregate to form the typical keratohyalin granules of the stratum granulosum. In addition, lipids are produced and stored in lamellar bodies. At the final stage of differentiation, the keratinocytes lose their organelles, including the nucleus, and become the dead, flattened corneocytes of the stratum corneum. During cornification, proteins are cross-linked at the inner side of the cytoplasmic membrane to form a cornified envelope (for review see Candi et al., 2005). In the transitional layer between the stratum granulosum and the stratum corneum, lipids are extruded to form a water-repelling envelope around the cornified envelope, thereby assuring an adequate permeability barrier function of the mammalian epidermis. Improper formation of these envelopes results in an impaired epidermal barrier that cannot protect against dehydration, UVB, and infection. Finally, corneocytes are shed from the skin by a process called desquamation. The signaling cascades involved in epidermal barrier formation are largely unknown, but the many proteases that seem to be involved are currently being intensively studied.
Since the cloning of caspase-14 in the late nineties, it has become clear that this protease is a unique member of the caspase family. Unlike apoptotic caspases, which evolved in common ancestors such as hydra, echinodermata, insects, nematodes, and chordates, caspase-14 has so far been found only in terrestrial mammals (Lamkanfi et al., 2002). In contrast to the ubiquitously expressed other members of the caspase family, caspase-14 is expressed and activated mainly in the epidermis and is absent from most other adult tissues (Eckhart et al., 2000b; Lippens et al., 2000). Recently, caspase-14 was found to be involved in epidermal barrier formation (Denecker et al., 2007). In this review, we discuss current knowledge of the expression, regulation, and function of caspase-14.
Caspase-14 expression and regulation
The expression pattern of caspase-14 is unique among the caspases, as it is present mainly in cornifying epithelia, such as the epidermis, the Hassall's bodies of the thymus, and the forestomach of rodents (Lippens et al., 2000, 2005; Denecker et al., 2007). In skin, caspase-14 is expressed only in the differentiating and cornifying layers of the epidermis and the hair follicle (Lippens et al., 2000; Alibardi et al., 2005). This is consistent with the observation that, in vitro, caspase-14 is only expressed in differentiating but not in proliferating keratinocytes (Lippens et al., 2000; Rendl et al., 2002). Remarkably, nail matrix keratinocytes that differentiate into specialized nail corneocytes, the building blocks of the nail plate, do not express caspase-14 (Jager et al., 2007). In addition, caspase-14 is not expressed in the noncornifying keratinocytes of the sweat gland or the mouth epithelium (Lippens et al., 2000; Alibardi et al., 2005). Ultrastructural analysis demonstrated that spatial distribution of caspase-14 in the epidermis and hair follicles is strongly conserved among several mammalian species (Alibardi et al., 2004, 2005). In the granular layer, caspase-14 was found to be associated with the nucleus, the keratohyalin granules, and the desmosomes, whereas in corneocytes, caspase-14 was found in the cytoplasm and was associated with corneodesmosomes (a modified version of desmosomes) and nuclear remnants. These observations suggested a role for caspase-14 in nuclear degradation during cornification, but nuclear degradation was not affected in caspase-14–deficient mice (Denecker et al., 2007). The expression of caspase-14 in the Hassall's bodies of the thymus and in the forestomach of rodents is somewhat expected, as they are cornifying structures and express the typical late differentiation markers, such as profilaggrin and loricrin, which are also found in the epidermis (Laster and Haynes, 1986; Favre, 1989; Jarnik et al., 1996). Protein expression of caspase-14 has also been reported in several noncornifying tissues (Lippens et al., 2003; Krajewska et al., 2004, 2005; Kam et al., 2005; Seidelin and Nielsen, 2006; Selicharova et al., 2007). However, these observations should be interpreted carefully, as we have recently shown that the reported expression of caspase-14 in such tissues can be the result of aspecific staining (Denecker et al., 2007).
Remarkably, so far caspase-14 has been found only in terrestrial mammals but not in birds or reptiles. Whereas birds and reptiles have a stiff, dry, scaly epidermis, mammals have a soft stratum corneum because of the larger amounts of histidine-rich late differentiation markers (e.g., profilaggrin; Alibardi, 2003). Interestingly, profilaggrin is a direct substrate of caspase-14 (Denecker et al., 2007). This could indicate that the occurrence of a soft stratum corneum and the caspase-14 gene are associated during evolution.
Although the expression of caspase-14 is very restricted, little is known about the transcriptional regulation of its gene. In vitro, caspase-14 is only expressed when keratinocytes are forced to differentiate by growing them postconfluently or in suspension or by adding vitamin D3 (Eckhart et al., 2000a; Lippens et al., 2000, 2004; Pistritto et al., 2002). In contrast, adding Ca2+ at high concentrations to the medium, a method frequently used to induce differentiation, did not induce caspase-14 expression (Eckhart et al., 2000a; Kuechle et al., 2001; Pistritto et al., 2002). Retinoids, which suppress keratinocyte differentiation, down-regulate caspase-14 expression (Rendl et al., 2002; Lippens et al., 2004).
These results indicate that transcription factors that are specifically active during terminal differentiation are required to regulate caspase-14 expression. Whether caspase-14 expression levels can be regulated at the posttranscriptional level is not known. Down-regulation of several differentiation-associated genes by retinoids has been shown to be mediated by the transrepression of activator protein 1 (AP-1)–mediated gene activation (Fisher and Voorhees, 1996). Indeed, the caspase-14 promoter contains at least two potential AP-1–binding sites (unpublished data). The green tea phenol (−)-epigallocatechin-3-gallate (EGCG) is a potent activator of AP-1 and has been shown to up-regulate caspase-14 in a p38- and JNK-dependent way (Hsu et al., 2005, 2007). AP-1 alone is probably not sufficient to drive caspase-14 expression because TNF and 12-O-tetradecanoyl-phorbol 13-acetate, two potent activators of AP-1 in keratinocytes (Arnott et al., 2002), did not induce caspase-14 expression in keratinocytes (Lippens et al., 2004).
Differentiation-dependent expression of caspase-14 in keratinocytes could also result from a strong transcriptional repression in proliferating keratinocytes. This possibility is supported by the observation that mice deficient in nuclear receptor corepressor Hairless (Hr) had 5–10-fold higher levels of caspase-14 and profilaggrin mRNA, starting from postnatal day 6 and progressing during development (Zarach et al., 2004). These alterations in gene expression were detected mainly in the keratinocytes of the utricle, an abnormal pouch-like structure at the upper part of the hair follicle. Increased gene expression occurred before the morphologically distinct utricle could be identified, indicating that the up-regulation was probably a cause rather than a consequence of utricle formation. It would be interesting to analyze both caspase-14 activation and filaggrin processing in these mice. Multiple mutant Hr alleles in mice and in humans show phenotypic variations that include congenital hair loss, skin wrinkling, and papular rash (Cichon et al., 1998; Panteleyev et al., 1998; Sprecher et al., 1998). Whether caspase-14 overexpression is important for the observed phenotypes could be addressed by generating epidermis-specific caspase-14 transgenic mice or by crossing the Hr mice with caspase-14–deficient mice.
Activation of caspase-14
Procaspases consist of a prodomain, a large subunit (p20), and a small subunit (p10). Activation of caspases is induced by dimerization, (auto-)proteolytic cleavage at Asp residues, and/or conformational changes (Lamkanfi et al., 2003). So far, maturation of caspase-14 by proteolytic cleavage into p20 and p10 subunits has been consistently observed only in cornifying epithelia such as the epidermis and the rodent forestomach (Lippens et al., 2000; Denecker et al., 2007). Although some investigators suggested that caspase-8 and -10 can activate caspase-14 in vitro (Ahmad et al., 1998; Van de Craen et al., 1998), this could not be confirmed by others (Lippens et al., 2000; Mikolajczyk et al., 2004). Furthermore, caspase-14 is probably not proteolytically activated by a caspase in vivo, as other caspases are not activated during epidermal differentiation (Eckhart et al., 2000b; Lippens et al., 2000; Raymond et al., 2007), and caspase-14 is not processed at an aspartate residue like other caspases but is processed at Ile152 in man and presumably at Leu167 in the mouse (Chien et al., 2002). Alignment of the protease-sensitive loop between the p20 and p10 subunits of the known mammalian procaspase-14 amino acid sequences (Fig. 2) reveals a conserved hydrophobic patch that is N terminal of the caspase-14 cleavage site. This patch contains P1-preferred amino acids of elastase-like serine proteases, such as Val, Ala, Leu, and Ile (Mallory and Travis, 1975; Vered et al., 1985; Takahashi et al., 1989), suggesting that a serine protease with elastase-like properties could be involved in caspase-14 activation (unpublished data).
All together, these data indicate that during skin homeostasis, the caspase-14–activating protease is not a caspase, separating caspase-14 activation from the apoptotic and inflammatory caspase cascades that could be detrimental to epidermal integrity. The precise epidermal layer in which caspase-14 is processed and activated is unknown because antiserum specifically recognizing activated caspase-14 is not yet available. However, the following findings indicate that activation of caspase-14 occurs at the interface between the granular and cornified layers of the epidermis or early during cornification. First, both the proform and activated form of caspase-14 can be found in total epidermal extracts, whereas in the cornified layer only activated caspase-14 is found (Fischer et al., 2004). Second, caspase-14 activation coincides with stratum corneum formation both during embryonic development and in organotypic skin cultures (Eckhart et al., 2000b; Lippens et al., 2004; Fischer et al., 2005). This implies that the main biological function of caspase-14 is exercised in the stratum corneum, as proven by the phenotype of the caspase-14–deficient mice (see the next section).
Identification of caspase-14 substrates has been hampered for a long time by the unavailability of enzymatically active caspase-14. However, it was recently shown that proteolytically processed caspase-14 requires high concentrations of kosmotropic salt to be active in vitro, such as sodium citrate, in addition to proteolytic cleavage between the p20 and p10 subunit (Mikolajczyk et al., 2004). These salts induce both dimerization and ordering of active site loops by partial desolvation of the protein to a more compact, catalytically active protease. The cellular environment of the stratum corneum of the epidermis probably favors caspase-14 activity in a similar way. Indeed, the water content decreases from 45% at the transitional layer to 15–25% at the skin surface (Warner et al., 1988; Caspers et al., 2001).
Surprisingly, human and murine caspase-14 have different substrate preferences. Human caspase-14 preferentially accommodates tryptophan or tyrosine in the S4 subsite, whereas mouse caspase-14 is more tolerant, with almost equal preferences for β-branched and aromatic amino acids (Mikolajczyk et al., 2004). For example, both human and mouse caspase-14 efficiently cleave the fluorescent peptide substrate WEHD-amc, but only mouse caspase-14 cleaves IETD-amc as efficiently (Fischer et al., 2004; Mikolajczyk et al., 2004). These substrate preferences would classify human caspase-14 as an inflammatory caspase and mouse caspase-14 as an inflammatory and apoptotic initiator caspase (Thornberry et al., 1997; Thornberry, 1998). However, human caspase-14 cannot proteolytically activate the inflammatory cytokines pro–interleukin-1β (our unpublished data) or -18 (Mikolajczyk et al., 2004), and there are no data supporting a direct role for caspase-14 in apoptosis (Lippens et al., 2000; Denecker et al., 2007). Whether the substrate preferences of human and murine caspase-14 observed in vitro on peptide substrates also occur in vivo is not clear. Importantly, profilaggrin, a major structural protein in the differentiating epidermis, has been shown to be a physiological substrate of caspase-14 (Denecker et al., 2007). Identification of additional substrates and determination of the cleavage sites will provide more insight into the preferred recognition sequence of caspase-14 in the context of a protein.
Function: caspase-14 is involved in cornification, hydration, and protection against UVB
Keratinocytes can die by two different processes: apoptotic cell death induced by damaging agents such as UVB, chemicals, and cytotoxic cytokines or by a continuous process of differentiation leading to the formation of corneocytes. These processes are clearly distinct pathways executed by different players (for review see Lippens et al., 2005), but the role of caspases in these two cell death programs has long been controversial. Although almost all procaspases are constitutively expressed in the epidermis, only caspase-14 has consistently been shown to be activated during epidermal cornification (Eckhart et al., 2000b; Lippens et al., 2000; Raymond et al., 2007). In addition, knockouts for apoptotic caspases were not reported to have a phenotypic skin anomaly except for caspase-3–deficient mice, in which keratinocyte differentiation is delayed in the embryo but normalized at birth (Okuyama et al., 2004). However, others could not confirm the activation of caspase-3 during embryonic epidermal development (Fischer et al., 2005). Although they are not activated during cornification, apoptotic caspases, in contrast to caspase-14, become activated during UVB-, staurosporine-, TNF-, and TNF-related apoptosis-inducing ligand–induced apoptosis of keratinocytes and, thereby, play a role in the apoptotic cell death of keratinocytes (for review see Lippens et al., 2005). Thus, we conclude that apoptotic caspases are not involved in the physiological keratinocyte cell death program leading to cornification. Furthermore, caspase-14–deficient epidermal cells can undergo classical apoptosis, which genetically demonstrates that caspase-14 is dispensable for the apoptosis of keratinocytes.
During development, caspase-14 protein expression is detectable from embryonic day (E) 15.5 on, and its processing is observed from E17.5 (Hu et al., 1998; Van de Craen et al., 1998; Fischer et al., 2005), which coincides with stratum corneum formation and establishment of the epidermal barrier. This indicates that caspase-14 might be involved in embryonic barrier formation. However, no differences in outside-in barrier formation of the skin of caspase-14–deficient mice during embryogenesis were observed (Denecker et al., 2007). Furthermore, caspase-14–deficient mice were born at the expected Mendelian ratios, were fertile, and had long survival rates. Detailed analysis of caspase-14–deficient mice indicated that caspase-14 has an important role in cornification, hydration, and UVB protection.
The skin of caspase-14–deficient mice was shinier, characterized by deeper skin lines, and had larger scales (Denecker et al., 2007) even though the shape and size of the cornified envelopes themselves were not altered. Biochemical analysis indicated that caspase-14 was responsible for the correct processing and degradation of (pro)filaggrin, as epidermis lacking caspase-14 was characterized by an altered profilaggrin processing and staining pattern (Fig. 3) and by the presence of aberrant keratohyalin granules, the profilaggrin storage granules.
Profilaggrin is a large, insoluble protein consisting of a calcium-binding A domain, a B domain, and several tandem repeats of filaggrin units. In the transitional layer, profilaggrin is dephosphorylated and proteolytically processed into its functional filaggrin units (Fig. 4), which aid in the bundling of keratin intermediate filaments and formation of the cornified envelope (for review see Candi et al., 2005).
Subsequently, filaggrin is deiminated (conversion of arginine to citrulline by elimination of the imino group of arginine by peptidylarginine deiminases), causing its release from keratin and allowing its degradation into free hygroscopic amino acids that act as natural moisturizing factors of the stratum corneum (Scott and Harding, 1986; Rawlings and Matts, 2005). Therefore, filaggrin plays an important role in skin hydration. Although the filaggrin unit was detected in caspase-14–deficient epidermis by Western blot analysis, lower molecular weight filaggrin fragments were also present (Denecker et al., 2007). Immunofluorescence analysis showed that in these mice, filaggrin immunoreactive fragments accumulated in the upper layers of the stratum corneum (Fig. 3). This indicates that the correct degradation of filaggrin into free amino acids was affected in caspase-14–deficient skin. Interestingly, caspase-14 was found to be associated with keratohyalin granules in the stratum granulosum and to remain cytoplasmic in the stratum corneum (Alibardi et al., 2004), which could correlate with its possible involvement in the generation of free amino acids.
These results, together with the finding that caspase-14 can directly cleave (pro)filaggrin in vitro, demonstrate that caspase-14 has a critical role in the correct processing of (pro)filaggrin during cornification. Whether the degradation of other differentiation-associated proteins is also affected in caspase-14–deficient mice remains to be determined. Two possible mechanisms for (pro)filaggrin processing by caspase-14 can be proposed (Fig. 4). First, caspase-14 may cleave the filaggrin fragments and, thereby, expose cleavage sites that can be recognized by other endo- and/or exopeptidases for further degradation. Second, caspase-14 may be the activator of an endo- and/or exopeptidase that cleaves and degrades filaggrin. This could occur directly, or indirectly by inactivating an inhibitor. Direct degradation of filaggrin fragments into free amino acids by caspase-14 can be ruled out, as caspases only cleave after aspartate residues.
The lack of filaggrin processing into free hygroscopic amino acids in caspase-14–deficient mice may lead to the reduced epidermal hydration and increased trans-epidermal water loss observed in these mice (Denecker et al., 2007). These results point to an important function of caspase-14 in the maintenance of epidermal hydration. Although a profilaggrin-deficient mouse has not been generated, it has been demonstrated that flaky tail (ft/ft) mice, which have an autosomal recessive mutation in the flaky tail gene (probably the profilaggrin gene), lack a functional filaggrin monomer (Presland et al., 2000). These mice have been proposed as a model for the filaggrin-deficient skin disease ichthyosis vulgaris because they have dry, flaky skin and irregular scales of variable size. The importance of filaggrin has been underscored recently by human genetic studies demonstrating that loss-of-function mutations in the profilaggrin gene are the primary cause of the skin disease ichthyosis vulgaris (Smith et al., 2006), which is characterized by silvery scales on the abdomen and palmar hyperlinearity. These mutations strongly predispose to atopic dermatitis and asthma (Palmer et al., 2006), possibly as a result of a defect in epidermal barrier function that allows the increased entry of allergens and infectious agents.
Two of the important functions of the skin are prevention of water loss and protection against environmental stress, such as protection against UVB radiation, which are essential for terrestrial life. The development of caspase-14–deficient mice revealed that the absence of caspase-14 enhances sensitivity toward UVB-induced photo damage and apoptosis of the skin (Denecker et al., 2007). Importantly, this is not caused by cell-autonomous differences in DNA damage sensitivity and apoptosis between wild-type and caspase-14–deficient keratinocytes. Instead, the UVB-filtering capacity of the stratum corneum is severely reduced in caspase-14–deficient skin, as higher levels of cyclobutane pyrimidine dimers are detected immediately after UVB irradiation. This indicates that caspase-14 has an indispensable role in the photoprotective function of the stratum corneum. Interestingly, topical application of EGCG, an inducer of caspase-14 expression, has been shown to be photoprotective (Elmets et al., 2001). How caspase-14 alters the structural and biochemical properties of the stratum corneum is currently under investigation.
Caspase-14 and disease
Caspase-14 was shown to be expressed at the protein level in several cancer cell lines (Pistritto et al., 2002; Koenig et al., 2005; Krajewska et al., 2005). In addition, caspase-14 mRNA, together with keratin 1 and profilaggrin mRNA, was decreased in murine UVB-induced squamous cell carcinoma, possibly reflecting reduced differentiation in the tumor (Rundhaug et al., 2005). Furthermore, in some cases, caspase-14 protein expression was associated with highly differentiated cornified areas of lung squamous cell carcinoma and cervix carcinoma (Koenig et al., 2005). However, caspase-14 activation in tumors has not been shown, and so it might not be responsible for the tumor phenotype. Presumably, the ectopic caspase-14 expression is caused by the changed transcriptional activity in these epithelial tumors. Mutations in the caspase-14 gene have not been found in human carcinomas except very rarely in colorectal tumors, which most probably were not the cause of altered caspase-14 expression (Koenig et al., 2005; Yoo et al., 2007).
We as well as other investigators demonstrated that caspase-14 expression is substantially down-regulated in psoriatic lesions but is unaffected in the nonlesional epidermis (Lippens et al., 2000, 2004; Walsh et al., 2005). Psoriasis is an autoimmune disease characterized by the uncontrolled proliferation of keratinocytes and impaired cornification, which results in the aberrant presence of nuclei in the cornified layer, also called parakeratosis. Although caspase-14 is absent in these parakeratotic regions, this is probably not the cause of the development of parakeratotic plaques, as caspase-14–deficient mice did not show spontaneous parakeratosis (Denecker et al., 2007). More likely, caspase-14 down-regulation results from the impairment of terminal differentiation or up-regulation of transcriptional repressors. The absence of caspase-14 in psoriatic plaques may lead to the formation of a defective barrier and, therefore, to the aggravation of psoriatic lesions. Treating the parakeratotic plaques of patients with a vitamin D3 analogue results in the up-regulation of caspase-14 and coincides with amelioration of the lesions (Lippens et al., 2004). Likewise, in the flaky skin (fsn/fsn) mouse model of psoriasis, topical EGCG treatment causes the up-regulation of caspase-14 and the amelioration of psoriasis (Hsu et al., 2007). Interestingly, the expression of JunB is strongly down-regulated in psoriatic lesions, and inducible epidermal deletion of both JunB and c-Jun in mice results in a psoriatic phenotype (Zenz et al., 2005). Because caspase-14 might be regulated by these transcription factors, it would be interesting to elucidate whether caspase-14 is down-regulated in JunB/c-Jun–deficient mice.
The role of caspase-14 has long been enigmatic. Recent evidence sheds light on the crucial role of caspase-14 in the skin, but several major questions remain. Activation of caspase-14 occurs most probably at the interface between the granular and cornified layer, implicating a role for caspase-14 in the stratum corneum. It is now clear that the caspase-14–activating protease is not a caspase but probably an epidermis-specific serine protease with elastase-like properties. This is not surprising, as it has been known for a long time that serine proteases are of major importance in epidermal homeostasis. Identification of the caspase-14–activating protease remains a major challenge. Determination of the in vitro conditions for caspase-14 activity that mimic stratum corneum conditions, together with the generation of caspase-14–deficient mice, led to the identification of (pro)filaggrin as the first known physiological caspase-14 substrate. Importantly, caspase-14 seems to be involved in the correct processing of filaggrin preceding its degradation into free hygroscopic amino acids, which might explain its role in the prevention of water loss from the epidermis. Proteomic approaches could lead to the identification of additional caspase-14 substrates, which would contribute to understanding the role of caspase-14 in the skin. Caspase-14 also protects against UVB-induced damage, which means that it is involved in the establishment of the biochemical or structural properties of the stratum corneum as a UVB filter. How caspase-14 establishes the UVB-filtering capacity of the corneum is not completely understood. An extensive biochemical analysis of caspase-14–deficient epidermis could reveal these mechanisms. Whether caspase-14, its activating protease, or its substrates could be used as therapeutic agents or as targets to improve formation of the epidermal barrier is a challenging research goal.
We thank A. Bredan for editing the manuscript, S. Lippens, E. Hoste, and L. Eckhart for fruitful discussions, and J. Verspurten for computational sequence analysis.
This work was supported by the Flanders Institute for Biotechnology as well by the European Community (EC) Marie Curie Training and Mobility Program, ApopTrain (grant MRTN-CT-035624), the EC Integrated Project, Epistem (grant LSHB-CT-2005-019067), Interuniversity Attraction Pole 6/18, and the Fonds voor Wetenschappelijke Onderzoek Vlaanderen (grants 3G.0218.06 and G.0133.05). This work was also supported by Ghent University grant BOF-GOA-12.0505.02 and by a Ghent University–cofinanced European Union project (grant I/00001/02). G. Denecker was a postdoctoral fellow at the Fonds voor Wetenschappelijk Onderzoek Vlaanderen; P. Ovaere was supported by an Emmanuel van der Schueren grant and an IWT predoctoral grant.
G. Denecker and P. Ovaere contributed equally to this paper.
Abbreviations used in this paper: AP-1, activator protein 1; EGCG, (−)-epigallocatechin-3-gallate.