How outer leaflet plasma membrane components, including glycosyl-phosphatidylinositol–anchored proteins (GPIAPs), transmit signals to the cell interior is an open question in membrane biology. By deliberately cross-linking several GPIAPs under antibody-conjugated 40-nm gold particles, transient anchorage of the gold particle–induced clusters of both Thy-1 and CD73, a 5′ exonucleotidase, occurred for periods ranging from 300 ms to 10 s in fibroblasts. Transient anchorage was abolished by cholesterol depletion, addition of the Src family kinase (SFK) inhibitor PP2, or in Src-Yes-Fyn knockout cells. Caveolin-1 knockout cells exhibited a reduced transient anchorage time, suggesting the partial participation of caveolin-1. In contrast, a transmembrane protein, the cystic fibrosis transmembrane conductance regulator, exhibited transient anchorage that occurred without deliberately enhanced cross-linking; moreover, it was only slightly inhibited by cholesterol depletion or SFK inhibition and depended completely on the interaction of its PDZ-binding domain with the cytoskeletal adaptor EBP50. We propose that cross-linked GPIAPs become transiently anchored via a cholesterol-dependent SFK-regulatable linkage between a transmembrane cluster sensor and the cytoskeleton.
The general signaling mechanisms by which the cross-linking of membrane determinants induces linkage to the cytoskeleton is a long-standing issue dating back to the original patching and capping observations (Raff et al., 1970) and the ideas of Singer (Singer, 1977; Holifield et al., 1990). More recently, such attachments have assumed clearer physiological and pathological importance. For example, receptor-induced dimerization (Lidke et al., 2005) causes retrograde transport off the filopodia to distal sites for further processing. Bead-induced clustering of integrins and cell adhesion molecules causes retrograde transport of these molecules away from the leading edge, and considerable effort has been devoted to the manner by which different sized ligand-coated beads induce clusters of cell adhesion molecules to link to the retrograde actin flow (Felsenfeld et al., 1996; Suter et al., 1998; Suter and Forscher, 2001). After binding to membrane receptors, viral particles are eventually associated with the cytoskeleton in different ways (Pelkmans et al., 2002; Ewers et al., 2005). T cell activation, which is initiated by ligation, is mediated by T cell receptor–containing microclusters that reorganize in an actin-dependent manner (Yokosuka et al., 2005).
Even lipids and glycosyl-phosphatidylinositol–anchored proteins (GPIAPs), when cross-linked, undergo patching and capping (Schroit and Pagano, 1981; Holifield et al., 1990), and GPIAPs can signal across the plasma membrane. The binding of antibody to several GPIAPs was shown early on to induce an association with Src family kinases (SFKs; Stefanova et al., 1991). Cross-linking the GPIAP Thy-1 on T lymphocytes results in mitogenesis (Kroczek et al., 1986; Zhang et al., 1992). Group B coxsackieviruses begin the process of infection of epithelial cells by binding to and clustering the GPIAP coreceptor decay-accelerating factor on the apical surface (Coyne and Bergelson, 2006). Transmembrane signaling has been speculated to occur in nanodomains such as lipid rafts when clusters are induced via receptor ligation and cross-linking (Simons and Toomre, 2000), and such signaling may serve to link the cluster to the cytoskeleton (Kusumi et al., 2004). However, the precise mechanisms of how GPIAPs signal and link to the cytoskeleton remain to be elucidated. This issue remains central in the study of the functionality of membrane microdomains (Kusumi et al., 2004).
In this study, we use a novel feature of single-particle tracking (SPT) trajectories as an assay to begin a dissection of how the linkage of certain GPIAPs and transmembrane proteins to the membrane-associated cytoskeleton may be regulated. SPT has been used to study membrane heterogeneity on various time and distance scales. Using video rate SPT, gold particles bound to membrane lipids and proteins were found temporarily corralled in transient confinement zones (TCZs; Simson et al., 1995; Sheets et al., 1997; Dietrich et al., 2002; Chen et al., 2004). With much higher time resolution, gold particles that bound to lipids and GPIAPs undergo compartmentalized hop diffusion on the millisecond time scale (Kusumi et al., 2005). Most previous experiments were aimed at producing pauci- or univalent gold to minimize the number of membrane molecules bound to gold so as to minimize artifacts caused by cross-linking membrane molecules (Murase et al., 2004). In contrast, in this study, we deliberately used the gold particle to form clusters of GPIAPs, mimicking the clusters formed under physiological conditions. The size of clusters associated with gold particles is much smaller than the size of clusters that were seen by immunostaining in previous studies (i.e., patches), which may represent ∼1,000 molecules (Holifield et al., 1990; Mayor et al., 1994). This protocol produced a unique nanoscale signature in the SPT trajectories, termed transient anchorage, that depends on SFKs, PI3 kinase, cholesterol, and caveolin-1. In some respects, our study confirms and extends the findings of Suzuki et al. (2004) using the GPIAP CD59. A transmembrane protein, the cystic fibrosis transmembrane conductance regulator (CFTR), also exhibits transient anchorage that strictly depends on its C-terminal PDZ-binding domain, but it is regulated differently than the GPIAP anchorage.
Mild cross-linking of membrane molecules by paucivalent gold is most likely the reason for transient confinement (Kusumi et al., 2004; Murase et al., 2004). However, in our hands, this type of transient confinement was not sensitive to inhibitors of SFKs (unpublished data), which is in contrast to results reported by Kusumi et al. (2004). We reasoned that perhaps our level of cross-linking was insufficient to induce the involvement of SFKs. Therefore, we used three different layers of cross-linking antibodies (Fig. 1 a) to collect the GPIAPs of interest together under and proximate to a single gold particle.
The trajectories of these clusters on the cell membrane reveal how signal transduction influences the lateral motion of GPIAPs that are sufficiently cross-linked.
We chose to investigate two GPIAPs, Thy-1 and CD73 (a 5′ exonucleotidase), both of which are endogenously expressed. The trajectories of both molecules exhibited a surprising feature: a considerable number of temporary anchorage events could be observed in the video record (Fig. 1 b and Video 1). To automatically identify the transient anchorage, a detection program was developed. Transient anchorage, in which particles stop (no displacement within experimental error) transiently, is distinguished from transient confinement, in which single particles still diffuse but in a slower and confined manner (Dietrich et al., 2002). The periods of transient anchorage varied from several hundred milliseconds to >10 s as shown in an example from cross-linked CD73 on IMR 90 cells and are bimodally distributed (Fig. 1 c). The longer anchorage times are directly visible in the video record. Fig. 2 a shows how the total relative confinement, combining both TCZs and transient anchorage for both Thy-1 (top) and CD73 (bottom), was increased by the maximal cross-linking protocol up to seven times compared with the control (left), in which the tertiary cross-linking antibody was not applied.
Note that despite different definitions of transient anchorage and transient confinement, transient anchorage points sometimes overlapped with TCZs. Therefore, to avoid the overestimation of relative confinement time (RCT) + relative anchorage time (RAT), the time segments in the trajectory in which transient anchorage was detected were removed, and the trajectory was concatenated. This operation resulted in a decreased RCT in trajectories in which transient anchorage was detected.
Transient anchorage only occurred after the addition of the tertiary antibodies (Fig. 1 a, bottom right) and showed titration behavior (Table I) so that there was an optimum concentration at a dilution of ∼1:100 with the final concentration of 20 μg/ml.
The dependence of transient anchorage on the concentration of tertiary antibody suggests that a critical size and/or number of cross-linked GPIAPs is required for transient anchorage (Harder and Simons, 1999). Having too many tertiary antibodies will result in a competition for the available binding sites offered by the primary antibodies; this will lead to monovalent binding of the tertiary antibody with diminished cross-linking and transient anchorage.
Transient anchorage also occurs with preassembled complexes: estimate of cluster size
The number of molecules cross-linked in one cluster is important for constructing a detailed mechanism of transient anchorage. In the maximal cross-linking protocol, primary antibodies are first bound to specific GPIAPs (Fig. 1); antibiotin gold is then added, decorating the surface at a very low concentration. Finally, tertiary antibody is added. Under these conditions, it is difficult to estimate the size of the cluster aggregated under one particle. In addition, the possibility of distal patches of cross-linked GPIAPs globally affecting the behavior of the gold particles cannot be excluded.
Therefore, we preassembled gold particle–antibody complexes to test whether transient anchorage would still occur. These complexes contain antibiotin gold with biotinylated anti–mouse IgG antibodies and anti–Thy-1 antibodies. An approximation to the number of primary antibodies bound to each gold particle in the preassembled complexes was made by estimating the contact area between the gold particle and cell membrane (see Gold conjugation to cells, procedure II). The estimate suggests that the maximal number of gold-bound Thy-1 molecules is likely to be <135 and is probably much less. These preassembled complexes exhibited transient anchorage, although the RAT was somewhat reduced (20%) compared with the original protocol (28%; Fig. S2 b). The bimodal distribution of anchorage times was similar to that seen in the maximal cross-linking SPT experiments (Fig. 1 d). Thus, a cluster of <135 GPIAPs is sufficient to induce transient anchorage.
Regulation of transient anchorage
Role of cholesterol.
To examine the role of cholesterol, which is found enriched in detergent-resistant membranes and is hypothesized to be an essential component of rafts, in transient anchorage, we performed cholesterol depletion before the maximal cross-linking. SPT trajectories obtained from treated cells were compared with the control. Transient anchorage was not detected in cells depleted of cholesterol by M CD (Thy-1 and CD73; Fig. 2 a, top and bottom, respectively) or removed by cholesterol oxidase (CD73; Fig. 2 a, bottom). Both Thy-1 and CD73 showed reduced transient confinement upon cholesterol depletion, which is consistent with previous studies (Sheets et al., 1997; Dietrich et al., 2001).
Role of SFKs.
GPIAPs such as CD59 and Thy-1 are found to couple with SFKs, which are distributed in the inner leaflet of the cell membrane, to effect signal transduction (Stefanova et al., 1991). It has also been shown that filamentous actin accumulates under patches of GPIAPs (Harder and Simons, 1999). The actin enrichment requires the activities of Fyn and Lck kinases (Harder and Simons, 1999), both of which belong to the Src family. A plausible hypothesis is that SFKs mediate the process of tethering the cross-linked GPIAP clusters to the membrane-associated cytoskeleton. Indeed, a form of transient confinement with the GPIAP CD59 has been shown to be mediated by Lck (Kusumi, 2004). Thus, we treated cells with the specific SFK inhibitor PP2 before the cross-linking. Transient anchorage was completely suppressed for both GPIAPs tested (Fig. 2 a), indicating that SFKs are critical for stabilization of the clusters. However, transient confinement was not substantially affected. Because transient anchorage occurs with preassembled particles, the possibility that global activation of SFKs by the maximal cross-linking protocol is responsible for transient anchorage is excluded.
We used the Src, Yes, and Fyn (SYF) triple knockout mouse fibroblast cells to confirm these results. Our anti–human CD73 antibody bound to CD73 on SYF cells, and Src rescued SYF cells as assessed by immunostaining (not depicted) and Western blots from these cells (Fig. 2 b). SYF defective cells showed no transient anchorage and reduced confinement similar to PP2-treated cells. However, transient anchorage was restored in Src-rescued SYF cells (Fig. 2 c). Thus, SFK activity is crucial for the transient anchorage phenotype.
Role of PI3 kinase.
Phosphoinositides PIP2 and PIP3 copatch with cross-linked raft components (Janes et al., 1999). It has also been shown that signaling via cross-linked GPIAP and choleratoxin-labeled cholesterol-containing clusters on the cell surface is blocked by both tyrosine kinase and PI3 kinase inhibition (Krauss and Altevogt, 1999), suggesting the involvement of PIP2 and PIP3 in signaling events induced by cross-linking. In addition, PIP2 and PIP3 found in inner leaflet microdomains are also involved in cytoskeletal regulation (Janmey and Lindberg, 2004). These findings suggest that changing the balance of PIP2 and PIP3 by inhibiting PI3 kinase might affect transient anchorage. To test this possibility, before SPT experiments, IMR 90 cells were incubated with the PI3 kinase inhibitor LY294004 for 15 min. Compared with control cells, PI3 kinase–inhibited cells exhibited more than a threefold greater transient anchorage (Fig. 3 a).
However, the proportion of the longer anchorage times was drastically reduced (compare Fig. 3 b with Fig. 1 c). Moreover, inspection revealed that half of the trajectories (23/47) exhibited bidirectional movements in the PI3 kinase–inhibited cells with excursions up to several micrometers (Fig. 3 c).
Role of caveolin.
Because cholesterol depletion also disrupts the structure of caveolae, which has been demonstrated by electron microscopy (Parpal et al., 2001; Dreja et al., 2002), a plausible possibility was that caveolae and caveolin might have a role in transient anchorage. Supporting this possibility, patches of cross-linked GPIAPs were found associated with caveolae (Mayor et al., 1994). By coimmunostaining of caveolin-1 and maximally cross-linked Thy-1 on C3H cells, we confirmed that cross-linked glycosyl-phosphatidylinositol–anchored Thy-1 partially colocalizes with caveolin-1 (Fig. 4, c and d).
Similar results were obtained for CD73 and caveolin-1 in IMR 90 cells (Fig. 4, a and b). In contrast, if cells were prefixed and subjected to maximal cross-linking, minimal colocalization was observed (unpublished data).To test the involvement of caveolin-1 in the transient anchorage phenotype, we used caveolin-1 knockout mouse embryonic fibroblasts. The expression of caveolin-1 was checked in knockout and control wild-type embryonic fibroblasts by immunostaining and confirmed the absence of caveolin-1 on the cell surface of the knockout cells but not the wild type (unpublished data). Also, both caveolin-1 wild-type cells and knockout cells express CD73 (Fig. 2 b). As shown in Fig. 4 c, the RAT was reduced from ∼45% in wild-type cells to ∼15% in caveolin-1 knockout cells. After PP2 treatment to inhibit SFKs, the RAT was reduced to <5% in both knockout and wild-type cells. A similar effect was observed when cholesterol depleted from both cell types. The result suggests that caveolin-1 contributes to the transient anchorage, although apparently other mechanisms are also involved, as indicated by the persistence of transient anchorage that is PP2 inhibitable in caveolin-1 knockout cells.
Transient anchorage exhibited by a transmembrane protein is regulated in a different manner
To further test whether the critical role of SFKs in the transient anchorage of GPIAPs is related to phosphorylation-enabled cytoskeletal association of clustered GPIAPs, we examined the diffusion behavior of a transmembrane protein, the CFTR. The C-terminal cytoplasmic domain of CFTR associates with the actin cytoskeleton via Na+/H+ exchanger regulatory factor PDZ proteins (EBP50 and E3KARP) and ezrin (Short et al., 1998; Sun et al., 2000; Raghuram et al., 2003). If transient cytoskeletal association is the cause of transient anchorage and cross-linking is necessary to recruit SFKs for clustered GPIAPs to be linked to the cytoskeleton, CFTR might be expected to demonstrate transient anchorage in a cross-linking and SFK-independent manner. Furthermore, if cholesterol involvement in the transient anchorage of GPIAPs is required because SFKs can only be recruited under the clusters through cholesterol-mediated nanodomains, CFTR might also be expected to demonstrate transient anchorage independent of cholesterol.
CFTR tagged with an extracellular HA epitope was expressed in C3H cells and labeled with biotinylated anti-HA antibodies and antibiotin antibody–conjugated gold. We found that CFTR displayed transient anchorage in the absence of tertiary cross-linking antibody (Fig. 5).
Upon PP2 or cholesterol depletion treatment, gold-conjugated HA-CFTR demonstrated only a modest reduction in RAT (Fig. 5), whereas both Thy-1 and CD73 showed a marked decrement in RAT after the same treatments (Fig. 2 a). Also, deletion of the PDZ-binding domain in CFTR, the Δ4 mutant (Gentzsch et al., 2004), caused transient anchorage to be almost completely eliminated (Fig. 5), indicating that Na+/H+ exchanger regulatory factor (EBP50) PDZ proteins and ezrin provide the key linkage to the cytoskeleton that is required for the transient anchorage of CFTR to occur. Thus, GPIAPs exhibit a much more marked dependence of transient anchorage on SFK and cholesterol than does transmembrane CFTR. This suggests a mechanism by which cross-linked GPIAPs could exploit (via a cholesterol-mediated nanodomain followed by SFK regulation) the normal linkages of transmembrane proteins to the cytoskeleton (see Discussion).
Robust transient anchorage was observed with the maximal cross-linking protocol. The preassembled complexes clustering 135 or fewer Thy-1 GPIAPs are shown to be sufficient to trigger transient anchorage comparable with the clusters formed by maximal cross-linking. Cholesterol is essential for gold particle–bound clusters to be transiently anchored on the cell surface, suggesting that a cholesterol-dependent nanodomain is formed under maximally cross-linked gold particles. The cross-linking–triggered signaling events involve SFK cascades, PI3 kinase, and caveolin-1. What molecular mechanisms might account for this phenomenon? The broad outlines of how different modes of transient anchorage might occur can be gleaned from recent reviews. Simons and Toomre (2000) suggested that the cross-linking of receptors might be a key to effecting signal transduction by either altering partitioning into existing raft domains or bringing smaller rafts together. Kusumi et al. (2004) suggested that one mode of coupling clustered GPIAPs may involve an unspecified transmembrane protein and recruitment of small inner leaflet rafts containing lipid-linked signaling molecules to the site of the cluster raft on the outer leaflet.
To focus future experiments, we propose a somewhat more specific working hypothesis (Fig. 6) using these ideas as a starting point.
In this model, cross-linking induces cholesterol-dependent nanodomains (cluster rafts) to form on both the inner and outer leaflet. Such clusters would include key transmembrane proteins bridging the inner and outer leaflets so that signals could be transmitted across the membrane in discrete locations. These proteins would be included from the outset and/or incorporated after collision of the cluster with the transmembrane protein. Whether initial anchoring is assisted by oligomerization-induced trapping (Kusumi et al., 2005) is an open issue. When an activated SFK randomly partitions into such a nanodomain on the inner leaflet, phosphorylation by SFK induces a resident transmembrane molecule to attach to the actin cytoskeleton through adaptor proteins. The attachment results in a transient anchorage event that continues until the SFK is deactivated and a recruited phosphatase dephosphorylates the resident linking molecule.
Specific molecular players can be accommodated by this generic hypothesis. A novel SFK substrate, C-terminal Src kinase–binding protein (Cbp; or PAG), was identified recently as a transmembrane protein-binding partner for C-terminal Src kinase, a kinase that regulates SFKs by phosphorylation at their C-terminal regulatory site (Matsuoka et al., 2004). There is also evidence indicating that Thy-1 is associated with Cbp (Durrheim et al., 2001). Furthermore, after phosphorylation by proximate SFKs, Cbp binds the ERM (ezrin/radixin/moesin)-binding protein (EBP50) via its PDZ domain and, thus, associates with the cytoskeleton through ERM proteins (Itoh et al., 2002). Therefore, Cbp is a plausible candidate for the transmembrane protein component of the hypothesis. Indeed, we have obtained results that are at least consistent with the transient anchorage of GPIAPs proceeding via the EBP50-ERM-actin cytoskeleton linkage: an EBP50-binding transmembrane protein, CFTR, exhibits transient anchorage without using the maximal cross-linking protocol, and removal of the C-terminal PDZ-binding domain of CFTR, which binds EBP50, in the Δ4 mutant (Gentzsch et al., 2004) abrogates transient anchorage (Fig. 5). Therefore, our hypothesis suggests that there is a common mode of cytoskeletal binding for both CFTR and GPIAPs (via EBP50-ezrin) but two different ways to couple to these adaptors, either directly (CFTR) or indirectly (GPIAP) via the formation of nanodomains and SFK regulation. Indeed, a regulatable ezrin linkage to Cbp was recently hypothesized to link rafts containing the B cell receptor to the lymphocyte cytoskeleton (Gupta et al., 2006).
Previous studies have shown that caveolin-1 deficiency diminishes both the number of caveolae (Drab et al., 2001) and caveolin-2 expression (Razani et al., 2001). Therefore, the diminution of transient anchorage in caveolin-1−/− cells is consistent with some of it occurring in caveolar structures. Alternatively or additionally, other cellular functions of caveolin-1 and -2 may be associated with transient anchorage. In their studies of the mobility of Simian virus 40 (SV40) on the cell surface before and just after its entry into the cell, Damm et al. (2005) also found a partial dependence on caveolin-1. Their findings can be compared and contrasted with our study. After diffusing, SV40 often stops as opposed to being transiently anchored as a prelude to internalization via caveolin-1–dependent or –independent pathways. However, only the caveolin-1–independent internalization pathway of SV40 requires tyrosine kinase activity and cholesterol.
In our study, transient anchorage does not exclusively depend on caveolin-1, suggesting at least two pathways to anchorage, but both pathways depended on cholesterol and SFK. Damm et al. (2005) also used SPT to study the diffusion behavior of mouse polyoma viruslike particles (which are 45 nm in diameter and similar to the size of gold particles) bound to live cell membranes and found cholesterol-dependent but SFK-independent confinement (Ewers et al., 2005). Viruslike particles exhibited confined diffusion in small zones 30–60 nm in diameter, which differs from transient anchorage in the fact that neither SFKs nor caveolin is required for confinement. Confinement requires the cross-linking of viral receptor (ganglioside), which promotes linkage to the actin cytoskeleton in an as yet undefined way. In general, based on the extensive study of viral interactions with the cell membrane, it could be anticipated that multiple modes of transient anchorage would exist (Marsh and Helenius, 2006).
Unexpectedly, inhibition of the conversion of PIP2 into PIP3 by PI3 kinase enhances transient anchorage and induces directional motion in a substantial fraction of trajectories. It is possible that PIP2 may preferentially partition into the inner leaflet microdomains induced by the cross-linked clusters of GPIAPs, and transient anchorage might then be formed via PIP2 to adaptor proteins that are associated with the actin cytoskeleton underneath the cell membrane, such as the ERM family proteins (Fievet et al., 2004). The bidirectional movements observed in some of the trajectories suggests that PIP2 clusters that participate in transient anchorage might also bind to motor proteins through their FERM or pleckstrin homology domains, thus contributing to the directed transport of cross-linked clusters by walking along cytoskeletal filaments. Binding between clustered PIP2 and motor proteins also provides a possible explanation for the disappearance of longer stopping periods because the motor proteins are active most of the time and might be expected to move the cluster directionally with short pauses (Kural et al., 2005).
Collectively, our data suggests that the transient anchorage phenotype may be regulated in different ways depending on the biological context. Moreover, the transient anchorage assay presented here should be valuable in defining precise linkages to the cytoskeleton and how they are regulated.
Materials And Methods
C3H 10T1/2 mouse fibroblasts (American Type Culture Collection [ATCC]) and IMR 90 human fibroblasts were maintained in basal medium Eagle and DME, respectively, and both were supplemented with 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. 2–4 d before an SPT experiment, fibroblasts were plated onto sterile 22 × 22-mm coverslips (glass) that were placed into 35-mm petri dishes at an appropriate cell density that yielded single cells for SPT measurements. Cells were depleted of cholesterol by treatment with 5 mM MβCD (Sigma-Aldrich) at 37°C for 30 min in unsupplemented medium or with 0.5 units of cholesterol oxidase (C8273; Sigma-Aldrich) at 37°C for 1 h in complete medium, respectively. The Src−/−, Yes−/−, and Fyn−/− mouse embryonic cell line (SYF; ATCC) and the SYF cell line with restored c-Src expression by the retroviral vector pLXSH (SYF+; ATCC) were used to examine the effect of SFKs on transient anchorage. The caveolin-1–deficient mouse embryonic cell line (caveolin-1−/−) and its wild-type parental cell line, which were gifts from M. Schwartz (University of Virginia, Charlottesville, VA), were used for experiments investigating the role of caveolin-1 in transient anchorage. HA-tagged CFTR protein was expressed in C3H cells by LipofectAMINE (Invitrogen) transfection.
Gold conjugation to cells
Antibiotin mouse IgG gold (BBInternational) was dissolved in Ham's F-12 nutrition mixture (Life Technologies/Invitrogen) supplemented with 25 mM Hepes and 15% serum (HHS), sedimented by centrifugation for 15 min at 11,000 g at 4°C, and resuspended in HHS. Cells on the coverslips were first incubated with biotinylated primary antibodies (1.5 mg/ml monoclonal mouse anti–Thy-1 antibody [clone 19EX5] or 0.15 mg/ml monoclonal mouse anti–human CD73 antibody [clone AD2; a gift from L. Thompson, Oklahoma Medical Research Foundation, Oklahoma City, OK]) for 10 min and were washed three times with 1 ml HH (HHS without serum). Then, the coverslip was mounted to form an open chamber on the slide with spacers on two parallel edges of the coverslip. 100 μl of the solution containing antibiotin gold (4.5 × 109 particles/ml) was injected into the chamber and incubated with cells for 10 min at 37°C. Unbound gold particles were removed by washing with 100 μl HH. 100 μl of the solution containing tertiary antibodies (0.02 mg/ml goat anti–mouse IgG; Zymed Laboratories) for the purpose of maximal cross-linking was injected into the chamber at this time if required. A final washing step with 100 μl of injected HHS was performed to remove unbound gold. For SPT of CFTR, 0.1 mg/ml biotinylated mouse anti-HA antibody (clone 16B12; Covance) was used as the primary antibody followed by the addition of antibiotin gold. SPT was then performed without the addition of cross-linking tertiary antibodies. For all experiments, after the gold particle incubations, the sample chamber was sealed with wax and mounted on the microscope. Particle trajectories were recorded for the following 30 min at 37°C and were maintained by an air curtain incubator.
Procedure II (preassembled complexes).
Gold particles conjugated with antibodies specific to GPIAPs of interest were assembled before addition to the cells. Preassembled complexes (Fig. S2 a) were made by mixing 20 μl antibiotin gold (4.5 × 1011 particles/ml), 2 μl biotinylated goat anti–mouse IgG antibodies (2 mg/ml), and 20 μl mouse anti–Thy-1 antibodies (15.5 mg/ml) in HHS solution with a final volume of 2 ml. The preassembled complexes were then spun down, resuspended, and added to the cell membrane for SPT experiments as described in the previous paragraph for procedure I. On the basis of the number of antibiotin conjugated to gold (150 antibodies/particle) as specified by the manufacturer (BBInternational), the biotinylated goat anti–mouse IgG antibodies (2.6 × 10−11 mol) added were in 10-fold excess over the antibiotin antibodies coated on gold, and the mouse anti–Thy-1 antibodies (10−9 mol) added were in 40-fold excess over the goat anti–mouse IgG antibodies.
Colocalization of cross-linked GPIAPs with caveolin-1.
Cross-linking of GPIAPs was accomplished by incubating cells with biotinylated primary monoclonal antibodies followed by antibiotin gold (BBInternational) and AlexaFluor488 polyclonal goat anti–mouse IgG antibody (Invitrogen) as in procedure I in the Gold conjugation to cells section. Cells were fixed with a mixture of 2% PFA and 2.5% glutaraldehyde for 10 min, washed three times with PBS, and permeabilized with 1% NP-40. Rabbit anti–mouse caveolin-1 antibody (Santa Cruz Biotechnology, Inc.) and Texas red–labeled goat anti–rabbit IgG antibody (Oncogene Research Products) were used to stain the caveolin-1 inside the cell. After extensive washing in PBS, cells on the coverslips were mounted on the slide with FluorSave reagent (Calbiochem) and observed using a fluorescence microscope (IX-81; Olympus) with a 100× objective. The fluorescent images of fixed samples were taken by a dual-mode cooled CCD camera (C4880; Hamamatsu) with MetaMorph image acquisition software (Molecular Devices) at room temperature. As a negative control in immunostaining, cells were prefixed in the 2% PFA and 2.5% glutaraldehyde mixture, after which the maximal cross-linking protocol was used; under such conditions, clustering of the GPIAPs would not be expected.
Time-lapse gold imaging for SPT
Computer-enhanced video microscopy, which was described previously (Lee et al., 1991), was used to image colloidal gold bound to the plasma membrane of fibroblast cells. In brief, the cell lamella with bound gold was imaged in brightfield mode and recorded with a video camera (Newvicon; Hamamatsu). After real-time background subtraction and contrast enhancement with an image processing unit (Argus 20; Hamamatsu), video frames were recorded in time-lapse mode (1,800 frames with 30 frames/s) on the hard disk of a computer (O2; Silicon Graphics). Recorded videos were analyzed by the commercial software package ISee (Inovision Corp.), which identifies relative changes of gold particle positions on the cell lamella with a precision of ±20 nm. All trajectories were visually inspected to ensure the correct tracking of gold particles.
Trajectories of gold particles obtained from SPT videos were analyzed for both transient confinement and transient anchorage. The detection for TCZs was performed as described previously (Simson et al., 1995). Transient anchorage detection software was designed to detect regions of trajectories where no displacement within experimental error occurred, as defined by all aspects of measurement stability. By recording videos of gold particles firmly glued on coverslips using nail polish and then detecting particle centroids over the time recorded, it was determined that 95% of centroids fell within ±25 nm along both the x and y axis (Fig. S1). A program was developed to recognize fragments from a trajectory as potential transient anchorage events when the displacement in successive frames was <25 nm in both the x and y dimensions. To qualify as transient anchorage, this condition must have persisted for more than four video frames (132 ms). Note that transient anchorage differs from immobilization, in which the particle does not move during the experimental observation time of a particular cell. To further characterize transient anchorage, we defined the RAT as the sum of durations in which a single particle is transiently anchored/total time of trajectory. To avoid the overestimation of total confinement given that transient anchorage sometimes overlaps with TCZs, transient anchorage segments were removed from the trajectories, and the remaining trajectory fragments were concatenated before TCZ detection.
Estimation of the maximum number of GPIAPs bound to preassembled complexes
We calculated this estimate using the following considerations. Gold particles almost appear to float on the membrane and can diffuse rapidly. This suggests that they are not nearly enveloped by the plasma membrane. The degree to which they can bend the membrane around them will determine the maximum number of membrane antigens the preassembled complex can bind. The bending modulus determines how facilely the membrane can be molded. However, the bending modulus will depend on the length scale examined. For example, if the particle is over an actin or spectrin filament, bending will not be as easy as if the particle is in the region between filaments. The maximum bending may be estimated in the following way. Let us say the compartment dimension of the cytoskeleton meshwork underlying the membrane is ∼100 nm. As shown in Fig. S2 a, taking the thickness of a single layer of antibodies to be 10 nm (Kienberger et al., 2004), the overall preassembled complex diameter would maximally be 100 nm. For the sake of argument, say each membrane (bilayer plus peripheral proteins) is ∼10 nm thick, so the gold particle complex in which a membrane partially wraps it has a radius of 60 nm. The furthest the membrane-wrapped particle could sink between adjacent filaments (Fig. S2 a) would result in the membrane covering the gold–antibody complex with a coverage area (CA), given by the equation
where α represents the half angle corresponding to the contact area and r represents the radius of the whole complex (Fig. S2 a). For the geometrical situation depicted in Fig. S2 a, α ≈ 56°. Assuming each binding site is saturated by the antibodies added in the next layer, the maximal number of surface Thy-1 bound to the particle is calculated as
In equation 2, the number of available anti–Thy-1 antibodies on the particle was multiplied by two because of the bivalency of antibodies (total number bound = 600). Given that r = 60 nm and 1 − cosα ≈ 0.45, the maximum number of bound Thy-1 was calculated as being ≤135. Considering the possible inactivation by partial denaturation of antibiotin antibodies during the coating process on gold particles, the steric and orientation considerations preventing antibody binding, and whether the particle actually sinks to the extent postulated in calculation, the actual number of Thy-1 bound to the preassembled complexes could be much less.
In equilibrium, the free energy gained from the antibody–antigen interaction should be greater than or equal to the energy required to bend the membrane to conform the membrane to the particle (Ebend). To bend an area of the membrane with an effective radius of curvature of 60 nm will require an energy given by
where kc represents the bending modulus of the cell membrane, r is the radius of curvature, and the area is given by the calculation in equation 1 (Sackmann, 1994). The local bending modulus will most likely lie somewhere between that for a bilayer, in which kc ≈ 2 8 kBT at 37°C (Waugh et al., 1992), and that for the red cell membrane, in which kc ≈ 23kBT (Evans, 1980). This leads to Ebend values ranging from 40 kBT, if pure bilayer intervened between the cytoskeletal filaments (Sako and Kusumi, 1995), to 33 kBT, if the red cell membrane modulus is more appropriate. The energy released from antibody–antigen bond formation ranges from 11 to 22 kBT at 37°C, corresponding to Ka values from 105 and 1010 M−1 (Van Regenmortel, 1998). Therefore, the free energy released from only four or fewer antibody–antigen bonds would be sufficient to bend the membrane to the extent required by the aforementioned extreme model.
While this paper was being reviewed, a study concluded that so much energy is gained from ligand–receptor bonds that the plasma membrane would completely envelope a 100-nm–diameter HIV particle (Sun and Wirtz, 2006). Our calculation assumes that the complete envelopment of a coated 40-nm gold particle would be frustrated by the presence of immediately subjacent cytoskeletal filaments. Then, between transient anchorage points, gold particles also diffuse rapidly, which seems unlikely if they were completely enveloped.
Image acquisition descriptions
In Fig. 4 (a–d), we used a microscope (IX-81; Olympus) with a 100× NA 1.25 oil immersion objective (Olympus). FluorSave reagent (Calbiochem) was used as the imaging medium, and AlexaFluor488 (Fig. 4, a and c) and Texas red (Fig. 4, b and d) were used as the fluorochromes. Images were captured with a dual-mode cooled CCD camera (C4880; Hamamatsu) and MetaMorph image acquisition software (Molecular Devices). Photoshop (Adobe) was used to crop the whole cell images into small fractions.
Online supplemental material
Fig. S1 shows determination positional noise in the SPT system. Fig. S2 shows that preassembled complexes bound to ≤135 GPIAPs still demonstrate transient anchorage. Video 1 shows that the gold-bound cross-linked Thy-1 cluster is diffusing on the C3H membrane with transient anchorage.
We thank G. Schuetz for helpful discussions.
This work was supported by the National Institutes of Health (grant GM 41402 to K. Jacobson) and Cystic Fibrosis Foundation (grant to S.L. Milgram).
Abbreviations used in this paper: Cbp, C-terminal Src kinase–binding protein; CFTR, cystic fibrosis transmembrane conductance regulator; GPIAP, glycosyl-phosphatidylinositol–anchored protein; RAT, relative anchorage time; RCT, relative confinement time; SFK, Src family kinase; SPT, single-particle tracking; SV40, Simian virus 40; TCZ, transient confinement zone.