page 637). The finding further unites the centrosome and primary cilia, both of which are based around centrioles, and both of which use pericentrin as an anchor for other proteins.
The authors show that pericentrin is located not only at centrosomes but also around the centrioles at the base of primary and motile cilia. After depletion of most pericentrin using RNAi, primary cilia failed to assemble, but centrosomes could still nucleate microtubules. The divergent outcomes may result from selective depletion of pericentrin's large isoform by RNAi, with remaining smaller isoforms perhaps acting in centrosomes.
Depletion of pericentrin also disrupted the localization of both the intraflagellar transport (IFT) proteins that help build cilia and PC2, a cation channel important in ciliary signaling. Furthermore, depletion of IFT proteins with RNAi caused pericentrin mislocalization and disrupted primary cilia formation.
Endogenous pericentrin copurifies and coimmunoprecipitates with IFT proteins. Additional studies are needed to understand exactly how this complex acts to direct primary cilia formation. The association is certainly reminiscent of pericentrin's function in centrosomes, where it is needed to anchor the γ-tubulin ring complexes that nucleate microtubules. ▪