Now, Young et al. (page 629) get the structural ball rolling with an EM characterization of yeast cortical actin patches, which are the most prominent actin structures in budding yeast. They find significant structural parallels between the yeast and mammalian systems, suggesting that the smaller yeast structures may be a handy model for tackling problems of actin dynamics.
The team partially purified patches from cells expressing GFP-labeled capping protein. Once cells were lysed, the researchers stabilized and cross-linked the actin patches and used correlated fluorescence and electron microscopy to visualize them.
Actin filaments were arrayed in a branched fashion, with the branch placement and angles characteristic of Arp2/3 complex–induced branching seen in mammalian cells. The authors conclude that a modified form of the dendritic nucleation model, which is used to explain actin dynamics at the leading edge of motile mammalian cells, does apply to yeast actin dynamics. The relevance of this model to yeast had been questioned because the concentration of actin protein in yeast appeared to be too low to support dendritic nucleation.
The yeast actin patches are known to move as they drive endocytosis. In the isolated patches, the branching was equal in all directions, but patches isolated directly from the cortex of living cells (both wild type and mutant) may reveal what process provides directionality. ▪