Vol. 165, No. 3, May 10, 2004. Pages 357–369.
This article originally appeared with errors in the legend to Fig. 7. The cyclohexamide (CHX) concentration should be 10 μg/ml, not 10 g/ml as published. The correct text is printed below.
Figure 7. Effect of antioxidants and protein synthesis inhibitor on neuronal degeneration and ERK/caspase-3 activation. At the time of potassium change (low K+), 5 mM N-acetyl cystein (N-AC), 50 U/ml superoxide dismutase (SOD), or 10 μg/ml cyclohexamide (CHX) was added; and triple staining and Western blot analysis were performed at the indicated time points. (A) The triple staining for the aforementioned groups at 24 h. ***P < 0.001, **P < 0.01, and *P < 0.05 compared with untreated cultures. (B) Western blot probed for total ERK (ERK), p-ERK, and caspase-3. (C) 5 mM N-AC, 50 U/ml SOD, and 10 μg/ml CHX were added immediately after potassium change, and cell extracts were assayed for caspase-3 activation; —, untreated; blank, without cell extract. Error bars represent mean ± SEM from at least six experiments. (D) CHX does not act by binding to ERK and caspase-3. 10 μg/ml CHX was added at 0 and 12 h after the potassium switch. 20 μM U0126 was added at 12 h. The cell lysates were isolated after 13 h and processed for caspase-3 and ERK activation. Tubulin and total ERK blots reveal total protein loading.